文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

- 介导的结肠癌细胞增殖和扩散受miR-221-3p调控。

-mediated colon cancer cell proliferation and dissemination is regulated by miR-221-3p.

作者信息

Liu Xiaojian, Xing Chungen

机构信息

Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.

出版信息

Transl Cancer Res. 2019 Aug;8(4):1289-1300. doi: 10.21037/tcr.2019.07.12.

DOI:10.21037/tcr.2019.07.12
PMID:35116871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8798939/
Abstract

BACKGROUND: Suppression of expression in colon cancer tissues may be associated with elevated levels of the microRNA 221 (miR-211). To uncover potential targets for treatment, the present study investigated in colon cancer development, and explored the role of miR-221-3p in the regulation of . METHODS: RNA expression arrays were searched in the NCBI database, and (PDZ domain containing ring finger 4) was selected as a potential downregulated gene in colon cancer. mRNA and protein in colon cancer and matched normal tissues were analyzed. The proliferation and dissemination of HCT116 cells overexpressing was assessed via functional assays. Bioinformatics analysis and luciferase reporter assay were applied to determine the regulatory link between miR-221-3p and mRNA. RESULTS: There was significantly less mRNA and protein in colon cancer tissue compared with normal tissues. HCT116 cells overexpressing were less able to disseminate relative to the control. Expression of was directly inhibited by miR-221-3p. Knockout of miR-211-3p was associated with attenuated proliferation and dissemination of HCT116 cells. CONCLUSIONS: may function as a tumor suppressor and is downregulated in colon cancer tissues, possibly due to dysregulation via miR-221-3p. This study provides new insight into colon cancer development.

摘要

背景：结肠癌组织中[某基因]表达的抑制可能与微小RNA 221（miR - 221）水平升高有关。为了揭示潜在的治疗靶点，本研究调查了[某基因]在结肠癌发生发展中的作用，并探讨了miR - 221 - 3p在[某基因]调控中的作用。 方法：在NCBI数据库中检索RNA表达阵列，并选择[含PDZ结构域的环指蛋白4（PDZ domain containing ring finger 4）]作为结肠癌中潜在的下调基因。分析结肠癌组织和配对的正常组织中[该基因]的mRNA和蛋白。通过功能实验评估过表达[该基因]的HCT116细胞的增殖和扩散情况。应用生物信息学分析和荧光素酶报告基因实验来确定miR - 221 - 3p与[该基因]mRNA之间的调控联系。 结果：与正常组织相比，结肠癌组织中[该基因]的mRNA和蛋白显著减少。相对于对照组，过表达[该基因]的HCT116细胞扩散能力较弱。[该基因]的表达受到miR - 221 - 3p的直接抑制。敲除miR - 211 - 3p与HCT116细胞增殖和扩散减弱有关。 结论：[该基因]可能作为一种肿瘤抑制因子，在结肠癌组织中表达下调，可能是由于miR - 221 - 3p调控异常所致。本研究为结肠癌的发生发展提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed58/8798939/2a38fcd1a169/tcr-08-04-1289-fS.2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed58/8798939/6e8972a7045a/tcr-08-04-1289-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed58/8798939/2b563272d3f1/tcr-08-04-1289-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed58/8798939/e88e5ec093d7/tcr-08-04-1289-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed58/8798939/98082aacc003/tcr-08-04-1289-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed58/8798939/d0ab280e4bed/tcr-08-04-1289-fS.1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed58/8798939/2a38fcd1a169/tcr-08-04-1289-fS.2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed58/8798939/6e8972a7045a/tcr-08-04-1289-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed58/8798939/2b563272d3f1/tcr-08-04-1289-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed58/8798939/e88e5ec093d7/tcr-08-04-1289-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed58/8798939/98082aacc003/tcr-08-04-1289-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed58/8798939/d0ab280e4bed/tcr-08-04-1289-fS.1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed58/8798939/2a38fcd1a169/tcr-08-04-1289-fS.2.jpg

相似文献

[1]
-mediated colon cancer cell proliferation and dissemination is regulated by miR-221-3p.

Transl Cancer Res. 2019-8

[2]
Reduced PDZRN4 promotes breast cancer progression and predicts poor prognosis.

Int J Clin Exp Pathol. 2019-1-1

[3]
PDZRN4 suppresses tumorigenesis and androgen therapy-resistance in prostate cancer.

J Cancer. 2022-4-18

[4]
PDZRN4 acts as a suppressor of cell proliferation in human liver cancer cell lines.

Cell Biochem Funct. 2015-10

[5]
MiR-372-3p Functions as a Tumor Suppressor in Colon Cancer by Targeting MAP3K2.

Front Genet. 2022-3-30

[6]
miR-1307-3p overexpression inhibits cell proliferation and promotes cell apoptosis by targeting ISM1 in colon cancer.

Mol Cell Probes. 2019-9-9

[7]
MicroRNA-455-3p Inhibits Tumor Cell Proliferation and Induces Apoptosis in HCT116 Human Colon Cancer Cells.

Med Sci Monit. 2016-11-18

[8]
Study on effects of miR-141-3p in proliferation, migration, invasion and apoptosis of colon cancer cells by inhibiting Bcl2.

Clin Transl Oncol. 2021-12

[9]
Downregulated miR-585-3p promotes cell growth and proliferation in colon cancer by upregulating .

Onco Targets Ther. 2019-8-14

[10]
MicroRNA-299-3p suppresses proliferation and invasion by targeting VEGFA in human colon carcinoma.

Biomed Pharmacother. 2017-7-18

引用本文的文献

[1]
Research progress of nanocarriers for gene therapy targeting abnormal glucose and lipid metabolism in tumors.

Drug Deliv. 2021-12

本文引用的文献

[1]
IMP1 3' UTR shortening enhances metastatic burden in colorectal cancer.

Carcinogenesis. 2019-6-10

[2]
miR-29b negatively regulates MMP2 to impact gastric cancer development by suppress gastric cancer cell migration and tumor growth.

J Cancer. 2018-10-1

[3]
miRNA-21 and miRNA-223 expression signature as a predictor for lymph node metastasis, distant metastasis and survival in kidney renal clear cell carcinoma.

J Cancer. 2018-9-8

[4]
miR-221 regulates proliferation and apoptosis of ovarian cancer cells by targeting BMF.

Oncol Lett. 2018-11

[5]
MiR-221-3p is down-regulated in preeclampsia and affects trophoblast growth, invasion and migration partly via targeting thrombospondin 2.

Biomed Pharmacother. 2018-11-2

[6]
MicroRNA-221 promotes cell proliferation, migration, and differentiation by regulation of ZFPM2 in osteoblasts.

Braz J Med Biol Res. 2018-10-18

[7]
An overview of 25 years of incidence, treatment and outcome of colorectal cancer patients.

Int J Cancer. 2018-9-29

[8]
Diabetes, plasma glucose and incidence of colorectal cancer in Chinese adults: a prospective study of 0.5 million people.

J Epidemiol Community Health. 2018-7-3

[9]
Obesity as a determinant of perioperative and postoperative outcome in patients following colorectal cancer surgery: A population-based study (2009-2016).

Eur J Surg Oncol. 2018-6-6

[10]
Abnormal expression of mRNA, microRNA alteration and aberrant DNA methylation patterns in rectal adenocarcinoma.

PLoS One. 2017-3-28

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索