研究 miR-141-3p 通过抑制 Bcl2 对结肠癌细胞增殖、迁移、侵袭和凋亡的影响。

Study on effects of miR-141-3p in proliferation, migration, invasion and apoptosis of colon cancer cells by inhibiting Bcl2.

机构信息

Ward 1, Department of General Surgery, The Third Affiliated Hospital of Qiqihaer Medical University, No. 27 Taishun Street, Tiefeng District, Qiqihaer City, 161000, Heilongjiang Province, China.

Department of Stomatology, The Third Affiliated Hospital of Qiqihaer Medical University, Qiqihaer City, 161000, China.

出版信息

Clin Transl Oncol. 2021 Dec;23(12):2526-2535. doi: 10.1007/s12094-021-02653-2. Epub 2021 Jun 4.

DOI:10.1007/s12094-021-02653-2

PMID:34086253

Abstract

PURPOSE

This study aimed to investigate the relationship between miR-141-3p and B lymphocyte-2 gene (Bcl2) gene and its biological behavior on colon cancer cell line SW480.

METHODS

qRT-PCR was used to detect the expression level of miR-141-3p in colon cancer tissues and adjacent tissues, as well as in colon cancer cell line and normal human colonic epithelial cell line FHC. MTT assay, wound assay, and Transwell demonstrated the effects of miR-141-3p on colon cancer proliferation, migration and invasion. Targetscan7.1 predictive software and dual luciferase reporter assays were used to detect the targeted regulation of miR-141-3p on the apoptosis-related gene Bcl2. MTT assay, wound assay, Transwell and flow cytometry were used to detect the effect of Bcl2 on miR-141-3p on colon cancer proliferation, migration, invasion and apoptosis.

RESULTS

Compared with adjacent tissues, the expression of miR-141-3p in colon cancer tissues was significantly down-regulated. Colon cancer patients with low expression of miR-141-3p had poorer prognosis. Compared with normal colonic epithelial cells, miR-141-3p expression was significantly down-regulated in colon cancer cell lines, and overexpression of miR-141-3p significantly attenuated the proliferation, migration and invasion of colon cancer cells. Knockdown of miR-141-3p significantly promoted the proliferation, migration and invasion of colon cancer cells. miR-141-3p targets the negative regulation of Bcl2. Knockdown of Bcl2 significantly attenuated the promotion of miR-141-3p inhibitor on proliferation, migration and invasion of colon cancer cells and inhibition of apoptosis. Knockdown of Bcl2 significantly enhanced the inhibition effect of miR-141-3p inhibitor on proliferation, migration and invasion of colon cancer cells.

CONCLUSIONS

In conclusion, miR-141-3p can inhibit the cancer by regulating Bcl2, and miR-141-3p has the potential to become a potential therapeutic target for colon cancer.

摘要

目的

本研究旨在探讨微小 RNA-141-3p（miR-141-3p）与 B 淋巴细胞瘤-2 基因（Bcl2）之间的关系及其对结肠癌细胞系 SW480 的生物学行为的影响。

方法

采用 qRT-PCR 检测结肠癌组织及癌旁组织、结肠癌细胞系和正常结肠上皮细胞系 FHC 中 miR-141-3p 的表达水平。MTT 实验、划痕实验和 Transwell 实验检测 miR-141-3p 对结肠癌细胞增殖、迁移和侵袭的影响。Targetscan7.1 预测软件和双荧光素酶报告基因实验检测 miR-141-3p 对凋亡相关基因 Bcl2 的靶向调控作用。MTT 实验、划痕实验、Transwell 实验和流式细胞术检测 Bcl2 对 miR-141-3p 对结肠癌细胞增殖、迁移、侵袭和凋亡的影响。

结果

与癌旁组织相比，结肠癌组织中 miR-141-3p 的表达明显下调。miR-141-3p 低表达的结肠癌患者预后较差。与正常结肠上皮细胞相比，结肠癌细胞系中 miR-141-3p 的表达明显下调，过表达 miR-141-3p 可显著抑制结肠癌细胞的增殖、迁移和侵袭。下调 miR-141-3p 可显著促进结肠癌细胞的增殖、迁移和侵袭。miR-141-3p 靶向负调控 Bcl2。下调 Bcl2 可显著减弱 miR-141-3p 抑制剂对结肠癌细胞增殖、迁移和侵袭的促进作用及对细胞凋亡的抑制作用。下调 Bcl2 可显著增强 miR-141-3p 抑制剂对结肠癌细胞增殖、迁移和侵袭的抑制作用。