Chen Yongyi, Gong Wangang, Zhou Yun, Fan Runping, Wu Yuchen, Pei Wangwei, Sun Sufang, Xu Xiaohong, Jiang Huifen
Institute of Cancer Research and Basic Medical Sciences of Chinese Academy of Sciences, Hangzhou 310022, China.
Department of Clinical Lab, Cancer Hospital of University of Chinese Academy of Sciences, Hangzhou 310022, China.
Transl Cancer Res. 2020 Apr;9(4):2941-2950. doi: 10.21037/tcr.2020.03.25.
Gastric cancer (GC) is one of the most common malignant neoplasms and is the third leading cause of cancer-related death around the world. Metformin has been well reported to have an inhibitory effect on the growth of various cancers by regulating the expression of microRNAs (miRNAs). However, the specific miRNA(s) regulated by metformin in GC have not been identified. In this study, real-time reverse transcription polymerase chain reaction (RT-PCR) analysis indicated that miR-107 expression was up-regulated in metformin-treated SGC-7901 cells compared with untreated SGC-7901 and MGC803 cells. Amplification of miR-107 expression further reduced cell proliferation in metformin-treated GC cells. A bioinformatics analysis showed that mitogen-activated protein kinase 8 (MAPK8) was the common target of metformin and miR-107. MAPK8 expression is associated with immune cell infiltration in GC as well as overall GC patient survival. Our study demonstrates that miR-107 enhances the anti-cancer effects of metformin in GC tissues, which offers a novel strategy for the treatment of GC.
胃癌（GC）是最常见的恶性肿瘤之一，也是全球癌症相关死亡的第三大主要原因。已有充分报道称二甲双胍通过调节微小RNA（miRNA）的表达对各种癌症的生长具有抑制作用。然而，二甲双胍在胃癌中调节的具体miRNA尚未确定。在本研究中，实时逆转录聚合酶链反应（RT-PCR）分析表明，与未处理的SGC-7901和MGC803细胞相比，二甲双胍处理的SGC-7901细胞中miR-107表达上调。miR-107表达的增强进一步降低了二甲双胍处理的胃癌细胞的增殖。生物信息学分析表明，丝裂原活化蛋白激酶8（MAPK8）是二甲双胍和miR-107的共同靶点。MAPK8表达与胃癌中的免疫细胞浸润以及胃癌患者的总体生存率相关。我们的研究表明，miR-107增强了二甲双胍在胃癌组织中的抗癌作用，这为胃癌的治疗提供了一种新策略。
