A method for determining the intracellular distribution of enzymes in yeast provides no evidence for the association of hexokinase with mitochondria.

A simple procedure based upon the principle discovered by Dürr et al. (Arch. Microbiol. (1975) 105, 319-327) was used to measure the intracellular distribution of enzymes in S. cerevisiae grown under both glucose repression and derepression. No substantial hexokinase activity was found to be associated with cellular organelles. The result does not support the hypotheses that reversible binding of hexokinase to mitochondria is important in regulation of glycolysis and cell growth.