Takamatsu K, Uchida T, Okada Y
Biochem Biophys Res Commun. 1986 Jan 29;134(2):1015-21. doi: 10.1016/s0006-291x(86)80522-8.
Elongation factor 2 (EF-2) was purified from rat liver extracts by affinity chromatography using fragment A of diphtheria toxin as the ligand. Purified EF-2 has a molecular weight of 96,000 and isoelectric point of 6.6-6.8. The sequence of the nineteen N-terminal amino acid is Val-Asn-Phe-Thr-Val-Asp-Gln-Ile-Arg-Ala Ile-Met-Asp-Lys-Lys-Ala-Asn and the C-terminal amino acid is leucine. Purified rat EF-2 modified with ADP-ribose was injected into rabbits to prepare antibodies against EF-2. The anti-EF-2 antibodies can immunoprecipitate with EF-2 from various eukaryotic cells.
通过使用白喉毒素片段A作为配体的亲和色谱法从大鼠肝脏提取物中纯化延伸因子2（EF-2）。纯化的EF-2分子量为96,000，等电点为6.6 - 6.8。N端十九个氨基酸的序列为Val-Asn-Phe-Thr-Val-Asp-Gln-Ile-Arg-Ala Ile-Met-Asp-Lys-Lys-Ala-Asn，C端氨基酸为亮氨酸。将用ADP-核糖修饰的纯化大鼠EF-2注射到兔子体内以制备抗EF-2抗体。抗EF-2抗体可与来自各种真核细胞的EF-2进行免疫沉淀。
