Sturgill T W, Ray L B
Biochem Biophys Res Commun. 1986 Jan 29;134(2):565-71. doi: 10.1016/s0006-291x(86)80457-0.
High molecular weight muscle protein(s), present as a "doublet" (approximately 320 and approximately 290 kDa apparent molecular weight) in partially purified preparations of inhibitor-2 from rabbit skeletal muscle, and homogeneous bovine brain microtubule associated protein-2 are both in vitro substrates for a soluble insulin-stimulatable serine/threonine kinase in 3T3-L1 adipocytes. The high molecular weight muscle substrate "doublet" was specifically immunoprecipitated by affinity-purified anti-microtubule associated protein-2 antibody.
高分子量肌肉蛋白，在来自兔骨骼肌的部分纯化的抑制剂-2制剂中以“双峰”形式存在（表观分子量约为320 kDa和约290 kDa），以及均一的牛脑微管相关蛋白-2，都是3T3-L1脂肪细胞中一种可溶性胰岛素刺激的丝氨酸/苏氨酸激酶的体外底物。高分子量肌肉底物“双峰”被亲和纯化的抗微管相关蛋白-2抗体特异性免疫沉淀。
