Center of Health Management, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
Department of Nephrology, Institute of Nephrology and Urology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
Endokrynol Pol. 2022;73(1):81-86. doi: 10.5603/EP.a2021.0106. Epub 2022 Feb 4.
Metabolic syndrome (MetS) is a clinical syndrome with several characteristics. Steroid receptor RNA activator (SRA) is a long non-coding RNA (lncRNA), which can increase the expression of steroid receptor-dependent gene. This study aimed to explore the changes in metabolic parameters and the predictive value of the peripheral blood mononuclear cells (PBMCs) to SRA ratios as new indicators in subjects with and without MetS in southern China.
There were 81 participants (39 with MetS and 42 without MetS) in this cross-sectional study. The expression of lncRNAs in PBMCs was evaluated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The risks of SRA and PBMCs to SRA ratios contributing to the presence of MetS were estimated by univariate and multivariate logistic regression models. The area under the receiver (AUC) operating characteristic curve was employed to evaluate diagnostic accuracy.
MetS was positively correlated with cortisol, interleukin 6 (IL-6), white blood cell to SRA ratio (WTSR), lymphocyte to SRA ratio (LTSR), monocyte to SRA ratio (MTSR), and PBMC to SRA ratio (PTSR). A receiver operating characteristic (ROC) curve analysis was performed to assess the value of LTSR (OR: 0.722; p < 0.001) for predicting MetS. The area under the curve yielded a cut-off value of 0.483, with a sensitivity of 76.9% and a specificity of 71.4% (p < 0.001).
In summary, SRA in PBMCs may be an important biomarker of stress reaction and may play a role in vulnerability to MetS. Also, the lymphocyte to SRA ratio demonstrated high accuracy in the diagnosis of MetS.
代谢综合征（MetS）是一种具有多种特征的临床综合征。类固醇受体 RNA 激活物（SRA）是一种长链非编码 RNA（lncRNA），可以增加类固醇受体依赖性基因的表达。本研究旨在探讨代谢参数的变化以及外周血单个核细胞（PBMC）中 SRA 比值的预测价值，作为中国南方 MetS 患者和非 MetS 患者的新指标。
这是一项横断面研究，共有 81 名参与者（39 名 MetS 患者和 42 名非 MetS 患者）。通过定量逆转录聚合酶链反应（qRT-PCR）评估 PBMCs 中 lncRNA 的表达。采用单变量和多变量逻辑回归模型估计 SRA 和 PBMCs 与 SRA 比值对 MetS 存在的风险。采用受试者工作特征曲线（ROC）下面积评估诊断准确性。
MetS 与皮质醇、白细胞介素 6（IL-6）、白细胞与 SRA 比值（WTSR）、淋巴细胞与 SRA 比值（LTSR）、单核细胞与 SRA 比值（MTSR）和 PBMC 与 SRA 比值（PTSR）呈正相关。采用 ROC 曲线分析评估 LTSR（OR：0.722；p<0.001）预测 MetS 的价值。曲线下面积得出的截断值为 0.483，灵敏度为 76.9%，特异性为 71.4%（p<0.001）。
总之，PBMC 中的 SRA 可能是应激反应的重要生物标志物，可能在易患 MetS 中起作用。此外，淋巴细胞与 SRA 比值对 MetS 的诊断具有较高的准确性。
