Horton M A, Chambers T J
Br J Exp Pathol. 1986 Feb;67(1):95-104.
A series of monoclonal antibodies have been developed which react specifically with human osteoclasts in human fetal bone and a range of neoplastic and non-neoplastic bone lesions. We have examined whether such antibodies retain reactivity and specificity with osteoclasts isolated from the bone of newborn non-human mammals and embryonic chickens. Of seven osteoclast-specific monoclonal antibodies, three retain their reactivity with rabbit and two with guinea-pig; none react with mouse or rat osteoclasts; two antibodies detect avian osteoclasts. The extensive retention of some osteoclast antigens during phylogeny suggests that they might represent functionally important molecules in the regulation of osteoclast activity. Moreover, the existence of antibodies capable of defining multi- and mononuclear osteoclasts, from species where osteoclast function is well characterized, suggests possible experimental routes towards the isolation, and hence study of purified populations of osteoclasts and their precursors.
已开发出一系列单克隆抗体，它们能与人胎儿骨以及一系列肿瘤性和非肿瘤性骨病变中的人破骨细胞发生特异性反应。我们研究了此类抗体对从新生非人类哺乳动物和胚胎鸡的骨骼中分离出的破骨细胞是否仍具有反应性和特异性。在七种破骨细胞特异性单克隆抗体中，三种与兔破骨细胞保持反应性，两种与豚鼠破骨细胞保持反应性；没有一种与小鼠或大鼠破骨细胞发生反应；两种抗体可检测禽类破骨细胞。在系统发育过程中某些破骨细胞抗原的广泛保留表明，它们可能代表破骨细胞活性调节中功能重要的分子。此外，存在能够识别破骨细胞功能已得到充分表征的物种中的多核和单核破骨细胞的抗体，这为破骨细胞及其前体的纯化群体的分离以及研究提供了可能的实验途径。
