Athanasou N A, Hall P A, d'Ardenne A J, Quinn J, McGee J O
Nuffield Department of Pathology, University of Oxford, John Radcliffe Hospital, UK.
Br J Exp Pathol. 1988 Apr;69(2):309-19.
The immunohistochemical reactivity of a monoclonal antibody, anti-L-35, on a wide range of tissues is described. Anti-L-35 showed a high specificity for known and presumptive cells of the mononuclear phagocyte system including monocytes, sinus histiocytes, tangible body macrophages, interdigitating reticulum cells, Kupffer cells, alveolar macrophages, microglia, synoviocytes and Langerhans cells. Anti-L-35 also stained osteoclasts in fetal and adult bone including osteoclast-like giant cells of the giant-cell tumour of bone. Anti-L-35 did not react with any other cell type in the tissues screened apart from renal proximal tubular epithelium and megakaryocytes. This study has shown that L-35 is not restricted to activated T-cells, as previously reported, and provides further immunohistochemical evidence that monocytes, macrophages and osteoclasts contain common cellular antigens.
本文描述了一种单克隆抗体抗-L-35在多种组织上的免疫组化反应性。抗-L-35对单核吞噬细胞系统的已知细胞和推测细胞具有高度特异性，包括单核细胞、窦组织细胞、可触知体巨噬细胞、交错突网状细胞、库普弗细胞、肺泡巨噬细胞、小胶质细胞、滑膜细胞和朗格汉斯细胞。抗-L-35还可使胎儿和成人骨骼中的破骨细胞染色，包括骨巨细胞瘤中的破骨细胞样巨细胞。除肾近端肾小管上皮细胞和巨核细胞外，抗-L-35在所筛查的组织中不与任何其他细胞类型发生反应。本研究表明，L-35并不像先前报道的那样仅限于活化的T细胞，并提供了进一步的免疫组化证据，证明单核细胞、巨噬细胞和破骨细胞含有共同的细胞抗原。
