Markey G M, Alexander H D, Agnew A N, McConnell R E, Morris T C, Robertson J H, Crockard A D, Bridges J M
Br J Haematol. 1986 Feb;62(2):257-73. doi: 10.1111/j.1365-2141.1986.tb02929.x.
An immunoperoxidase technique has been used to identify and enumerate helper and suppressor T-cell subsets, as defined by reactivity with Coulter T4 and OKT8 monoclonal antibodies in 54 patients with B chronic lymphocytic leukaemia (B-CLL) and in the same number of matched controls. The ratio of T4+ to T8+ cells was significantly reduced in the B-CLL group as a whole (P less than 0.001) and in each stage of the three clinical staging systems. There was an increase in the median absolute level of T8+ cells in the whole CLL group (P less than 0.001). However, subdivision of the CLL group by clinical staging systems revealed a large group (28 patients) in which median T8+ cell levels were not raised and median T4+ cell levels were low (P less than 0.01). There was no significant decrease in T4+:T8+ ratio, increase in T8+ cells or decrease in T4+ cells with progression of clinical stage. Absolute numbers of E+ cells were significantly raised in all staging systems as were E+ T4- T8- cells (P less than 0.001). A significant alteration in either of these populations with progression of clinical stage was not present.
采用免疫过氧化物酶技术，根据与库尔特T4和OKT8单克隆抗体的反应性来鉴定和计数辅助性T细胞和抑制性T细胞亚群，研究对象为54例B细胞慢性淋巴细胞白血病（B-CLL）患者以及相同数量的匹配对照。在整个B-CLL组以及三种临床分期系统的各个阶段，T4+细胞与T8+细胞的比例均显著降低（P<0.001）。整个CLL组中T8+细胞的中位绝对水平有所升高（P<0.001）。然而，根据临床分期系统对CLL组进行细分后发现，有一大组（28例患者）的T8+细胞中位水平未升高，T4+细胞中位水平较低（P<0.01）。随着临床分期的进展，T4+:T8+比例无显著降低，T8+细胞无增加，T4+细胞无减少。在所有分期系统中，E+细胞的绝对数量以及E+T4-T8-细胞的绝对数量均显著升高（P<0.001）。随着临床分期的进展，这些细胞群体均未出现显著变化。
