Pekary A E, Turner L F, Hershman J M
Clin Chem. 1986 Mar;32(3):511-4.
We have evaluated a new immunoenzymatic assay for human thyrotropin involving three monoclonal antibodies (Abbott HTSH EIA) and compared the results with those of two conventional nonequilibrium double-antibody radioimmunoassay (RIA) methods: Clinical Assays' RIA and a research RIA (J Clin Endocrinol Metab 1975; 41:676). Mean values for thyrotropin in 100 euthyroid serum samples were similar in the Abbott and Clinical Assays methods, but both sets were significantly higher than those by the research RIA. By all methods, values for hypothyroid patients were clearly higher than values for euthyroid subjects. Results for hyperthyroid and euthyroid subjects were resolved slightly better with the research RIA than with the Abbott kit. The new Abbott assay was far more sensitive than either the Clinical Assays RIA or our research RIA. The correlation of results of the Abbott assay with those of the Clinical Assays and the research RIA exceeded 90% for samples from hypothyroid patients. The Abbott assay replaces radioisotope counting with spectrophotometric detection.
我们评估了一种新的人促甲状腺激素免疫酶测定法，该方法涉及三种单克隆抗体（雅培促甲状腺激素酶免疫测定法），并将结果与两种传统的非平衡双抗体放射免疫测定法（RIA）进行了比较：临床检验公司的RIA和一种研究用RIA（《临床内分泌与代谢杂志》1975年；41:676）。100份甲状腺功能正常血清样本中促甲状腺激素的平均值在雅培和临床检验公司的方法中相似，但这两组值均显著高于研究用RIA的结果。通过所有方法，甲状腺功能减退患者的值明显高于甲状腺功能正常受试者的值。研究用RIA对甲状腺功能亢进和甲状腺功能正常受试者结果的分辨略优于雅培试剂盒。新的雅培测定法比临床检验公司的RIA或我们的研究用RIA灵敏得多。对于甲状腺功能减退患者的样本，雅培测定法与临床检验公司和研究用RIA结果的相关性超过90%。雅培测定法用分光光度检测取代了放射性同位素计数。
