低补体血症性肾脏疾病组织对豚鼠补体的固定作用

Guinea pig complement fixation by tissues from hypocomplementaemic renal diseases.

作者信息

Ohi H, Seki M, Sakashita T, Hatano M

出版信息

Clin Immunol Immunopathol. 1986 Apr;39(1):93-101. doi: 10.1016/0090-1229(86)90208-4.

Abstract

Recently many studies have been done to identify complement pathway activation in renal tissue from patients with renal disease. We examined whether tissues obtained by renal biopsy from such patients would fix guinea pig complement. Nine out of 15 patients with systemic lupus erythematosus (SLE), 4 out of 7 patients with mesangiocapillary glomerulonephritis (MCGN), and 2 out of 7 cases with acute glomerulonephritis (AGN) fixed guinea pig C3. We found that tissues from 5 out of 9 guinea pig C3-positive SLE cases fixed guinea pig C4, while none of the guinea pig C3-positive tissues from patients with MCGN or AGN fixed guinea pig C4. These guinea pig C3-positive renal tissues were further studied for interaction with C4-deficient guinea pig serum, EDTA guinea pig serum, heated guinea pig serum, and EGTA Mg2+ guinea pig serum. The results indicated that activation of both the alternate and classical complement pathways occurred with tissues from patients with SLE, while activation of the alternate pathway occurred with MCGN and AGN. Results for tissues from AGN and MCGN patients indicated the presence of C3 convertase and protease which interacted with guinea pig C3.

摘要

最近，人们开展了许多研究以确定肾病患者肾组织中的补体途径激活情况。我们检查了通过肾活检从这类患者获取的组织是否会结合豚鼠补体。15例系统性红斑狼疮（SLE）患者中有9例、7例系膜毛细血管性肾小球肾炎（MCGN）患者中有4例以及7例急性肾小球肾炎（AGN）患者中有2例的组织结合了豚鼠C3。我们发现，9例豚鼠C3阳性的SLE病例中有5例的组织结合了豚鼠C4，而MCGN或AGN患者的豚鼠C3阳性组织均未结合豚鼠C4。对这些豚鼠C3阳性肾组织进一步研究其与C4缺陷豚鼠血清、EDTA豚鼠血清、加热豚鼠血清以及EGTA Mg2+豚鼠血清的相互作用。结果表明，SLE患者的组织同时激活了替代补体途径和经典补体途径，而MCGN和AGN则激活了替代补体途径。AGN和MCGN患者组织的结果表明存在与豚鼠C3相互作用的C3转化酶和蛋白酶。