Uff J S, Evans D J, Bartolotti S R
J Clin Lab Immunol. 1979 Feb;1(4):299-304.
The in vitro fixation of heterologous complement by cryostat sections of human renal biopsy material was studied to determine the mechanism of complement activation. Various types of guinea pig sera with different parts of the complement system inhibited were used, the fixation of complement being detected by direct immunofluorescence. Cases of idiopathic focal nephritis with mesangial IgA (mesangial IgA disease), Henoch-Schönlein purpura (HSP) and mesangio-capillary glomerulonephritis (MCGN) fixed complement by the alternative pathway alone and in systemic lupus erythematosus (SLE) both the classical and alternative pathways were involved. Only one of the seven cases of membranous glomerulonephritis fixed complement and this was by the classical pathway. After prior treatment with C3b inactivator, the in vitro complement fixation in mesangial IgA disease, HSP and MCGN was greatly reduced. In SLE it was slightly reduced and in membranous glomerulonephritis there was no change. This is a convenient method of studying the biological properties of complexes which is believed to reflect the in vivo behaviour of the tissue deposited complex.
研究了用人肾活检材料的低温切片进行异源补体的体外固定，以确定补体激活的机制。使用了抑制补体系统不同部分的各种类型豚鼠血清，通过直接免疫荧光检测补体的固定情况。伴有系膜IgA的特发性局灶性肾炎（系膜IgA病）、过敏性紫癜（HSP）和系膜毛细血管性肾小球肾炎（MCGN）病例仅通过替代途径固定补体，而在系统性红斑狼疮（SLE）中，经典途径和替代途径均参与其中。七例膜性肾小球肾炎病例中只有一例固定补体，且是通过经典途径。用C3b灭活剂预先处理后，系膜IgA病、HSP和MCGN中的体外补体固定显著减少。在SLE中略有减少，而在膜性肾小球肾炎中则无变化。这是一种研究复合物生物学特性的便捷方法，据信它能反映沉积在组织中的复合物在体内的行为。
