Oud P S, Haag D J, Zahniser D J, Ramaekers F C, Huysmans A C, Veldhuizen J A, Verheyen R H, Verrijp K, Broers J L, Herman C J
Cytometry. 1986 Jan;7(1):8-17. doi: 10.1002/cyto.990070103.
This paper describes a new automated system to prepare slides of cytological material from suspension. The system collects material on a filter tape by filtration and transfers it to glass slides by means of pressure-fixation. Using cervical cells as a model, results show that a well-defined cell number is evenly deposited over a standardized area, while a small number of cells is retained on the tape and a negligible number lost in the filtrate. Contamination is very small. Application of the system to other cytological material (fine needle aspirations, monolayer and cell suspension cultures, agar cultures, and isolated nuclei) is shown. In general, more than one slide can be made from one sample. Several histological staining procedures as well as immunofluorescence labeling protocols can be applied to the preparations obtained in this way. This system thus introduces a method that will standardize specimen preparation, is quick, saves operator time, and can be used for both diagnostic and research applications.
本文描述了一种新型自动化系统，用于从悬浮液中制备细胞学材料玻片。该系统通过过滤将材料收集在滤带上，并通过压力固定将其转移到载玻片上。以宫颈细胞为模型，结果表明，明确数量的细胞均匀沉积在标准化区域，少量细胞保留在滤带上，滤液中损失的细胞数量可忽略不计。污染非常小。展示了该系统在其他细胞学材料（细针穿刺抽吸物、单层和细胞悬浮培养物、琼脂培养物以及分离的细胞核）上的应用。一般来说，一个样本可以制作不止一张玻片。几种组织学染色程序以及免疫荧光标记方案都可应用于通过这种方式获得的制剂。因此，该系统引入了一种能够使标本制备标准化的方法，速度快，节省操作人员时间，可用于诊断和研究应用。
