Otto K, Höffken H, Soost H J
Anal Quant Cytol. 1979 Jul-Aug;1(2):127-35.
Components and evaluations of a new preparative procedure for automated high-resolution analysis of cervical samples are presented. This procedure is based on sedimentation velocity separation of samples with subsequent fractionation of the separation column and centrifugal deposition of suspended cells on coated glass slides. A system for specimen collection and mailing of suspended samples is described. A new type of glass slide designed for automated analysis is presented, and centrifugal buckets for cell deposition on a 6-sq-cm area are described. Experimental results with different kinds of coating substances for glass slides as well as different isopyknic media are discussed, and data for differential cell counts are graphically demonstrated. Looking at the diagnostic accuracy and economic feasibility of this system, the authors realize that preparations have to be evaluated quantitatively and that constraints of sample size and processing time have to be taken into consideration for further developments.
本文介绍了一种用于宫颈样本自动高分辨率分析的新制备方法的组成部分及评估。该方法基于样本的沉降速度分离，随后对分离柱进行分级，并将悬浮细胞离心沉积在涂覆的载玻片上。描述了一种用于收集和邮寄悬浮样本的标本系统。介绍了一种为自动分析设计的新型载玻片，并描述了用于在6平方厘米区域进行细胞沉积的离心桶。讨论了使用不同类型的载玻片涂层物质以及不同等密度介质的实验结果，并以图形方式展示了细胞分类计数的数据。考虑到该系统的诊断准确性和经济可行性，作者认识到必须对制备物进行定量评估，并且在进一步开发时必须考虑样本大小和处理时间的限制。
