Institute of Biology of Komi Scientific Centre of the Ural Branch of the RAS, Syktyvkar, Russian Federation, 167982.
Institute of Chemistry of Komi Scientific Centre of the Ural Branch of the RAS, Syktyvkar, Russian Federation, 167982.
Biogerontology. 2022 Apr;23(2):215-235. doi: 10.1007/s10522-022-09954-1. Epub 2022 Feb 5.
Honeysuckle Lonicera pallasii (Lonicera caerulea L.) is an excellent source of anthocyanins which have a number of health-promoting properties mainly associated with antioxidant and anti-inflammatory activities. Cyanidin-3-O-glucoside (C3G) is one of the most common anthocyanins naturally found in honeysuckle. The goal of the present study was to investigate antioxidant and anti-aging properties of Lonicera pallasii (Lonicera caerulea L.) extract (LE) and C3G using red blood cells (RBC) and Drosophila melanogaster models. LE and C3G treatment at a concentration of 100 μM induced enhancement of median and maximum lifespan up to 8%. LE and C3G supplementation at a concentration of 100 μM increased stress resistance up to 10%. The locomotor activity decreased during LE and C3G treatment in 4 and 6 weeks up to 52% in females. The integrity of the intestinal barrier was increased by 4% after LE treatment. These effects were accompanied by increased expression of Hif1 (pro-longevity gene) in response to C3G treatment and decreased expression of Keap1 (anti-longevity gene) after C3G and LE supplementation. RNA interference-mediated knockdown of Sirt6 completely abolished the positive effect obtained of LE and C3G supplementation in males which indicates that lifespan-extending effect is associated with Sirt6 activation. The experiments on the various in-vitro models (including radical scavenging activity and oxidative hemolysis of RBC demonstrated antioxidant and membrane-protective activities of LE and C3G. The present study indicates that Lonicera extract can prolong the lifespan and improve the healthspan of Drosophila model through biological and antioxidant activities.
忍冬（Lonicera pallasii）（忍冬 Lonicera caerulea）是花色苷的优质来源，花色苷具有多种促进健康的特性，主要与抗氧化和抗炎活性有关。矢车菊素-3-O-葡萄糖苷（C3G）是天然存在于忍冬中的最常见花色苷之一。本研究的目的是使用红细胞（RBC）和黑腹果蝇模型来研究忍冬（Lonicera caerulea）提取物（LE）和 C3G 的抗氧化和抗衰老特性。LE 和 C3G 以 100μM 的浓度处理可将中值和最大寿命分别提高 8%和 8%。LE 和 C3G 以 100μM 的浓度补充可将应激抗性提高 10%。在 4 周和 6 周的 LE 和 C3G 处理过程中，雌性的运动活性降低了 52%。LE 处理后肠道屏障的完整性增加了 4%。这些作用伴随着 C3G 处理后 Hif1（长寿基因）表达增加，C3G 和 LE 补充后 Keap1（抗长寿基因）表达减少。RNA 干扰介导的 Sirt6 敲低完全消除了 LE 和 C3G 补充对雄性获得的阳性影响，这表明寿命延长的作用与 Sirt6 的激活有关。在各种体外模型（包括自由基清除活性和 RBC 的氧化溶血）上的实验表明，LE 和 C3G 具有抗氧化和膜保护活性。本研究表明，忍冬提取物通过生物和抗氧化活性可以延长黑腹果蝇模型的寿命并改善其健康范围。
