Meisel H
FEBS Lett. 1986 Feb 17;196(2):223-7. doi: 10.1016/0014-5793(86)80251-4.
The in vivo formation of an opioid peptide (exorphin) derived from beta-casein has been proved for the first time. It was isolated from duodenal chyme of minipigs after feeding with the milk protein casein. The exorphin has been identified as a beta-casein fragment by end-group determinations and qualitative amino acid analysis of the purified peptide. This peptide, named beta-casomorphin-11, displayed substantial opioid activity in an opiate receptor-binding assay.
首次证实了源自β-酪蛋白的阿片肽（外啡肽）在体内的形成。在用乳蛋白酪蛋白喂养小型猪后，从其十二指肠食糜中分离出了该物质。通过对纯化肽的端基测定和定性氨基酸分析，已将该外啡肽鉴定为β-酪蛋白片段。这种名为β-酪蛋白吗啡-11的肽在阿片受体结合试验中表现出显著的阿片样活性。
