Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität Berlin, Königsweg 65, 14163 Berlin, Germany.
Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität Berlin, Königsweg 65, 14163 Berlin, Germany.
J Dairy Sci. 2022 Apr;105(4):3468-3476. doi: 10.3168/jds.2021-21257. Epub 2022 Feb 3.
The objective of this study was to evaluate the effect of the nonsteroidal anti-inflammatory drug transdermal flunixin meglumine (Finadyne Transdermal) on plasma cortisol, average daily weight gain, and standing and lying behavior of calves, when given at the time of disbudding combined with local anesthesia. A sedative was not used to minimize pharmacological interactions. Seventy-one female Holstein Friesian calves aged 13 ± 2 d, with an average weight of 48.9 ± 4.26 kg were enrolled in the study. All calves were randomly assigned to one of 3 treatment groups: (1) control group (CON, n = 27), (2) 1-flunixin group (1-FLU, n = 26) with a single administration of transdermal flunixin meglumine at disbudding, and (3) 2-flunixin group (2-FLU, n = 24) with 2 administrations of transdermal flunixin meglumine, the first treatment at disbudding and the second 6 h after disbudding. Although the CON group received a placebo, 1-FLU and 2-FLU received flunixin meglumine transdermally. To account for plasma cortisol changes due to manipulation and handling of the calves, a sham disbudding procedure was performed one week before disbudding took place. Sham disbudding was conducted by using a cold cautery dehorner applied to each horn bud for 10 s. Disbudding was performed in a similar way by using a hot cautery dehorner. Plasma samples were collected to measure the stress biomarker cortisol at 7 different time points. Body weights were measured 4 times in 2 wk. Standing and lying behavior was assessed via 3-dimensional accelerometer. During sham disbudding and disbudding mean plasma cortisol concentrations were 6.09 ± 2.5 ng/mL and 5.16 ± 2.8 ng/mL, respectively. Treatment tended to have an effect on plasma cortisol concentrations during sham disbudding and had an effect on plasma cortisol concentrations during disbudding. Plasma cortisol concentrations were affected by treatment 2 h after disbudding in comparison to CON group. Furthermore, there was a significant effect on plasma cortisol concentrations 6 h after disbudding in contrast to CON. A return to baseline plasma cortisol levels (initial concentrations) was not achieved in CON during disbudding. There was no statistical difference between average daily weight gain and the treatment procedure. Total lying time was not affected by treatment after disbudding. In conclusion, transdermal flunixin meglumine given at the time of disbudding combined with local anesthesia decreased concentrations of the stress biomarker cortisol, but a second dose 6 h after disbudding had no further effect on plasma cortisol levels.
本研究旨在评估非甾体抗炎药透皮氟尼辛美罗格（Finadyne Transdermal）在去角联合局部麻醉时对犊牛血浆皮质醇、平均日增重和站立与卧姿行为的影响。为了尽量减少药物相互作用，未使用镇静剂。71 头 13±2 日龄的荷斯坦弗里生雌性犊牛，平均体重 48.9±4.26kg，纳入本研究。所有犊牛随机分为 3 个治疗组：（1）对照组（CON，n=27），（2）1-氟尼辛组（1-FLU，n=26），于去角时单次给予透皮氟尼辛美罗格，（3）2-氟尼辛组（2-FLU，n=24），于去角时和去角后 6h 分别给予 2 次透皮氟尼辛美罗格。尽管 CON 组接受安慰剂，但 1-FLU 和 2-FLU 均接受透皮氟尼辛美罗格治疗。为了考虑因操作和处理犊牛而导致的血浆皮质醇变化，在去角前一周进行了假去角操作。假去角通过将冷电烙去角器应用于每个角芽 10s 来完成。去角操作与使用热烙去角器的方式类似。在 7 个不同时间点采集血浆样本以测量应激生物标志物皮质醇。在 2 周内 4 次测量体重。通过 3 维加速度计评估站立和卧姿行为。在假去角和去角时，平均血浆皮质醇浓度分别为 6.09±2.5ng/mL 和 5.16±2.8ng/mL。处理对假去角和去角时的血浆皮质醇浓度有影响。与 CON 组相比，去角后 2h 时处理对血浆皮质醇浓度有影响。此外，与 CON 相比，去角后 6h 时血浆皮质醇浓度有显著影响。在去角过程中，CON 组未恢复到基线血浆皮质醇水平（初始浓度）。平均日增重与处理程序之间无统计学差异。去角后，总卧姿时间不受处理影响。结论：去角联合局部麻醉时给予透皮氟尼辛美罗格可降低应激生物标志物皮质醇浓度，但去角后 6h 给予第二剂对血浆皮质醇水平无进一步影响。
