São Paulo State University (UNESP), School of Veterinary Medicine and Animal Science, Botucatu, Brazil.
São Paulo State University (UNESP), School of Veterinary Medicine and Animal Science, Botucatu, Brazil.
J Proteomics. 2022 Apr 15;257:104508. doi: 10.1016/j.jprot.2022.104508. Epub 2022 Feb 4.
This study aimed to characterize the proteome of spermatozoa and seminal plasma of 4 purebred dogs (Golden Retriever, Great Dane, Bernese Mountain Dog, and Maremmano-Abruzzese Sheepdog). The ejaculate of 13 dogs was collected, and sperm characteristics were subjectively evaluated. Seminal plasma and sperm cells were separated and prepared individually for mass spectrometry. Data were evaluated by univariate and multivariate statistical analysis. A total of 162 proteins were identified, 47 in spermatozoa, 109 in seminal plasma, and 6 in both samples. Serum albumin in spermatozoa and tubulin alpha-3E chain, acrosin binding protein, and tubulin alpha-3 chain in plasma seminal were statistically relevant. Serum albumin and acrosin binding protein improve the sperm capacitation, acrosome reaction, and seminal quality. The tubulin family proteins are related to structural cell organization and flagella movement, and their presence in seminal plasma may be related to sample handling. According to cluster formation, a high association was observed among Bernese Mountain Dog and Great Dane, Golden Retriever, and Maremmano-Abruzzese Sheepdog for sperm proteins. For seminal plasma proteins, Bernese Mountain Dog, Great Dane, and Maremmano-Abruzzese Sheepdog were related. Further studies on breed-specific proteins in the semen of purebred dogs need to be performed to clarify its fertility roles. SIGNIFICANCE: For the first time spermatozoa proteins of dogs are described. The comparison of spermatozoa and seminal plasma proteins of four purebred dogs were performed. These results supporting that differences in semen protein profile of different canine breeds exist, which can improve the biotechnologies of reproduction in this species.
本研究旨在描述 4 种纯种犬（金毛猎犬、大丹犬、伯恩山犬和马雷马牧羊犬）的精子和精浆的蛋白质组。收集了 13 只犬的精液，并对精子特征进行了主观评估。分别分离和制备精浆和精子细胞进行质谱分析。通过单变量和多变量统计分析评估数据。共鉴定出 162 种蛋白质，其中 47 种存在于精子中，109 种存在于精浆中，6 种存在于两种样本中。精子中的血清白蛋白和浆中的α-3E 微管蛋白、顶体酶结合蛋白和α-3 微管蛋白在统计学上是相关的。血清白蛋白和顶体酶结合蛋白可改善精子获能、顶体反应和精液质量。微管家族蛋白与细胞结构组织和鞭毛运动有关，其在精浆中的存在可能与样本处理有关。根据聚类形成，在精子蛋白中观察到伯恩山犬和大丹犬、金毛猎犬和马雷马牧羊犬之间存在高度关联。对于精浆蛋白，伯尔尼山犬、大丹犬和马雷马牧羊犬之间存在关联。需要对纯种犬精液中的种特异性蛋白质进行进一步研究，以阐明其在生育中的作用。意义：首次描述了犬的精子蛋白。比较了 4 种纯种犬的精子和精浆蛋白质。这些结果表明不同犬种的精液蛋白谱存在差异，这可以提高该物种的生殖生物技术。
