Lugli Gabriele Andrea, Longhi Giulia, Alessandri Giulia, Mancabelli Leonardo, Tarracchini Chiara, Fontana Federico, Turroni Francesca, Milani Christian, Di Pierro Francesco, van Sinderen Douwe, Ventura Marco
Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy.
GenProbio Srl, Parma, Italy.
Front Microbiol. 2022 Jan 21;12:790881. doi: 10.3389/fmicb.2021.790881. eCollection 2021.
Probiotic bacteria are widely administered as dietary supplements and incorporated as active ingredients in a variety of functional foods due to their purported health-promoting features. Currently available probiotic products may have issues with regards to their formulation, such as insufficient levels of viable probiotic bacteria, complete lack of probiotic strains that are stated to be present in the product, and the presence of microbial contaminants. To avoid the distribution of such unsuitable or misleading products, we propose here a novel approach named Probiotic Identity Card (PIC), involving a combination of shotgun metagenomic sequencing and bacterial cell enumeration by flow cytometry. PIC was tested on 12 commercial probiotic supplements revealing several inconsistencies in the formulation of five such products based on their stated microbial composition and viability.
由于其据称的促进健康特性，益生菌作为膳食补充剂被广泛使用，并作为活性成分添加到各种功能性食品中。目前市面上的益生菌产品在配方方面可能存在问题，例如活的益生菌数量不足、产品中声称含有的益生菌菌株完全缺失以及存在微生物污染物。为避免此类不合适或误导性产品的流通，我们在此提出一种名为“益生菌身份证”（PIC）的新方法，该方法结合了鸟枪法宏基因组测序和流式细胞术细菌细胞计数。对12种商业益生菌补充剂进行了PIC测试，结果显示其中5种产品基于其宣称的微生物组成和活力，在配方上存在若干不一致之处。
