Wang Jiajing, Hu Bo, Jing Yuliang, Hu Xiping, Guo Yue, Chen Jiankun, Liu Yuxi, Hao Jianhui, Li Wen-Xia, Ning Hailong
Key Laboratory of Soybean Biology, Ministry of Education, Key Laboratory of Soybean Biology and Breeding/Genetics, Ministry of Agriculture, Northeast Agricultural University, Harbin, China.
Suihua Branch of Heilongjiang Academy of Agricultural Science, Suihua, China.
Front Plant Sci. 2022 Jan 21;12:803820. doi: 10.3389/fpls.2021.803820. eCollection 2021.
Soybean is an important global crop for edible protein and oil, and plant height is a main breeding goal which is closely related to its plant shape and yield. In this research, a high-density genetic linkage map was constructed by 1996 SNP-bin markers on the basis of a recombinant inbred line population derived from Dongnong L13 × Henong 60. A total of 33 QTL related to plant height were identified, of which five were repeatedly detected in multiple environments. In addition, a 455-germplasm population with 63,306 SNP markers was used for multi-locus association analysis. A total of 62 plant height QTN were detected, of which 26 were detected repeatedly under multiple methods. Two candidate genes, and , involving in plant height were predicted by pathway analysis in the regions identified by multiple environments and backgrounds, and validated by qRT-PCR. These results enriched the soybean plant height regulatory network and contributed to molecular selection-assisted breeding.
大豆是全球重要的食用蛋白和食用油作物，株高是一个主要的育种目标，与植株形态和产量密切相关。本研究基于东农L13×合农60衍生的重组自交系群体，利用1996个SNP-bin标记构建了高密度遗传连锁图谱。共鉴定出33个与株高相关的QTL，其中5个在多个环境中被重复检测到。此外，利用一个包含63306个SNP标记的455份种质资源群体进行多位点关联分析。共检测到62个株高QTN，其中26个在多种方法下被重复检测到。通过多环境和背景鉴定区域的通路分析预测了两个与株高相关的候选基因，并通过qRT-PCR进行了验证。这些结果丰富了大豆株高调控网络，有助于分子选择辅助育种。
