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结合连锁作图和 BSA 定位鉴定大豆株高和主茎节数的 QTL 和候选基因。

Combined Linkage Mapping and BSA to Identify QTL and Candidate Genes for Plant Height and the Number of Nodes on the Main Stem in Soybean.

机构信息

College of Agriculture, Northeast Agricultural University, Harbin 150030, China.

Jilin Academy of Agricultural Sciences, Soybean Research Institute, Changchun 130033, China.

出版信息

Int J Mol Sci. 2019 Dec 19;21(1):42. doi: 10.3390/ijms21010042.

DOI:10.3390/ijms21010042
PMID:31861685
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6981803/
Abstract

Soybean is one of the most important food and oil crops in the world. Plant height (PH) and the number of nodes on the main stem (NNMS) are quantitative traits closely related to soybean yield. In this study, we used 208 chromosome segment substitution lines (CSSL) populations constructed using "SN14" and "ZYD00006" for quantitative trait locus (QTL) mapping of PH and NNMS. Combined with bulked segregant analysis (BSA) by extreme materials, 8 consistent QTLs were identified. According to the gene annotation of the QTL interval, a total of 335 genes were obtained. Five of which were associated with PH and NNMS, potentially representing candidate genes. RT-qPCR of these 5 candidate genes revealed two genes with differential relative expression levels in the stems of different materials. Haplotype analysis showed that different single nucleotide polymorphisms (SNPs) between the excellent haplotypes in and may be the cause of changes in these traits. These results provide the basis for research on candidate genes and marker-assisted selection (MAS) in soybean breeding.

摘要

大豆是世界上最重要的粮食和油料作物之一。株高（PH）和主茎节数（NNMS）是与大豆产量密切相关的数量性状。本研究利用“SN14”和“ZYD00006”构建的 208 个染色体片段代换系（CSSL）群体，对 PH 和 NNMS 进行了数量性状位点（QTL）作图。结合极端材料的分离群体分组分析法（BSA），鉴定到 8 个一致性 QTL。根据 QTL 区间的基因注释，共获得 335 个基因。其中 5 个基因与 PH 和 NNMS 相关，可能代表候选基因。对这 5 个候选基因的 RT-qPCR 分析显示，不同材料茎中的两个基因具有不同的相对表达水平。单倍型分析表明，和 之间优秀单倍型之间的不同单核苷酸多态性（SNP）可能是这些性状变化的原因。这些结果为大豆候选基因和标记辅助选择（MAS）的研究提供了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b5/6981803/07086f226c2b/ijms-21-00042-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b5/6981803/e5cf247f33c4/ijms-21-00042-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b5/6981803/67edb2d11455/ijms-21-00042-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b5/6981803/2542e3c12463/ijms-21-00042-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b5/6981803/07086f226c2b/ijms-21-00042-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b5/6981803/e5cf247f33c4/ijms-21-00042-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b5/6981803/67edb2d11455/ijms-21-00042-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b5/6981803/ae6b3813ec32/ijms-21-00042-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b5/6981803/2542e3c12463/ijms-21-00042-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b5/6981803/07086f226c2b/ijms-21-00042-g005.jpg

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