来自伊朗腹泻犬的犬细小病毒2型亚型的系统发育分析。
Phylogenetic analysis of canine parvovirus 2 subtypes from diarrheic dogs in Iran.
作者信息
Ghajari M, Pourtaghi H, Lotfi M
机构信息
Department of Clinical Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran.
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
出版信息
Iran J Vet Res. 2021 Fall;22(4):347-351. doi: 10.22099/IJVR.2021.40878.5925.
BACKGROUND
Canine parvovirus type 2 (CPV-2) causes gastroenteritis and leukopenia in dogs worldwide. They are three subtypes of CPV-2 including CPV-2a, CPV-2b, and CPV-2c. The distribution status of CPV-2 subtypes has been shown differences in many countries.
AIMS
The aim of the present study was detection and phylogenetic analysis of different subtypes of CPV-2 circulating in two provinces of Iran, Tehran and Alborz.
METHODS
CPV-2 was detected using 555 primer pairs in collected samples. Phylogenetic analysis of CPV-2 subtypes was done using sequencing of the partial length of gene.
RESULTS
Twenty-eight CPV-2 were detected using 555 primer pair. The sequences of isolates were deposited in the GenBank database. Phylogenetic analysis revealed that all CPV-2c subtype isolates had very high sequence identity to China and Zambia that form a distinct cluster.
CONCLUSION
In conclusion, this study revealed the emergence of all CPV-2 variants in dogs in Iran. Thus, the continual monitoring of CPV-2 in domestic dogs should be further conducted on a large scale to determine the predominant variants and their distributions in the country and to follow the dynamics of CPV-2 in the Middle East region of Asia.
背景
犬细小病毒2型（CPV-2）在全球范围内导致犬类患肠胃炎和白细胞减少症。CPV-2有三种亚型，包括CPV-2a、CPV-2b和CPV-2c。在许多国家，CPV-2亚型的分布状况已显示出差异。
目的
本研究的目的是检测在伊朗德黑兰和阿尔伯兹两省流行的不同CPV-2亚型并进行系统发育分析。
方法
使用555引物对在采集的样本中检测CPV-2。通过对部分基因长度进行测序对CPV-2亚型进行系统发育分析。
结果
使用555引物对检测到28株CPV-2。分离株的序列已存入GenBank数据库。系统发育分析表明，所有CPV-2c亚型分离株与中国和赞比亚的分离株具有非常高的序列同一性，形成一个独特的聚类。
结论
总之，本研究揭示了伊朗犬类中所有CPV-2变体的出现。因此，应进一步大规模持续监测家犬中的CPV-2，以确定该国主要的变体及其分布，并跟踪亚洲中东地区CPV-2的动态变化。
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