Mokhtari Mojgan, Rouhanizadeh Noushin, Hajialiasgar Shahla
Department of Clinical and Anatomical Pathology, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Neurology, Isfahan University of Medical Sciences, Isfahan, Iran.
J Res Med Sci. 2021 Nov 29;26:108. doi: 10.4103/jrms.JRMS_573_20. eCollection 2021.
Melanoma is one of the most invasive cutaneous cancers with characteristics such as rapid progression and distant metastasis. The early diagnosis and staging of melanoma can help better manage the patients. The current study is aimed to assess the values of microRNA-10b (miRNA-10b) in the discrimination of metastatic melanomas.
The current cross-sectional study has been conducted on forty patients diagnosed with melanoma since 2011. Cell culture of melanoma cell lines derived from the cancerous tissue, including WM115, BLM, K1735, WM793, and A375M, was cultured. In order to assess miRNA-10b levels, the real-time polymerase chain reaction was utilized. The absence ( = 20)/presence ( = 20) of metastasis was diagnosed with chest computed tomography or chest X-ray. The values of miRNA-10b for the discrimination of metastasis incidence were assessed.
The demographic characteristics, including age and gender of the metastatic and nonmetastatic patients, were similar ( > 0.05). The specimen cultures were positive for miRNA-10b in 14 (35%) of the metastatic cases versus 4 (20%) of the nonmetastatic ones ( = 0.004). The quantitative analysis of miR-2b revealed significantly higher levels in metastatic cases (-1.59 ± 1.13 in metastatic vs. -0.16 ± 0.67 in nonmetastatic cases; = 0.001). The measured area under the curve for the value of miRNA-10b was 0.923 ( < 0.001; 95% confidence interval: 0.811-1) with sensitivity and specificity of 100% and 94.4%.
Based on this study, metastatic melanoma was associated with elevated levels of miRNA-10b. This marker had the sensitivity and specificity of 100% and 94.4% for the discrimination of metastatic melanoma from nonmetastatic ones.
黑色素瘤是侵袭性最强的皮肤癌之一，具有进展迅速和远处转移等特征。黑色素瘤的早期诊断和分期有助于更好地治疗患者。本研究旨在评估微小RNA - 10b（miRNA - 10b）在鉴别转移性黑色素瘤中的价值。
本横断面研究对2011年以来确诊的40例黑色素瘤患者进行。培养了源自癌组织的黑色素瘤细胞系的细胞培养物，包括WM115、BLM、K1735、WM793和A375M。为评估miRNA - 10b水平，采用了实时聚合酶链反应。通过胸部计算机断层扫描或胸部X线诊断有无转移（转移组 = 20例/非转移组 = 20例）。评估miRNA - 10b鉴别转移发生率的价值。
转移组和非转移组患者的人口统计学特征，包括年龄和性别相似（P > 0.05）。标本培养中，14例（35%）转移病例的miRNA - 10b呈阳性，而非转移病例为4例（20%）（P = 0.004）。miR - 2b的定量分析显示转移病例中的水平显著更高（转移组为 - 1.59 ± 1.13，非转移组为 - 0.16 ± 0.67；P = 0.001）。miRNA - 10b值的曲线下面积为0.923（P < 0.001；95%置信区间：0.811 - 1），敏感性和特异性分别为100%和94.4%。
基于本研究，转移性黑色素瘤与miRNA - 10b水平升高有关。该标志物鉴别转移性黑色素瘤与非转移性黑色素瘤的敏感性和特异性分别为100%和94.4%。
