Bai Ming, Zhang Hailin, Si Loubin, Yu Nanze, Zeng Ang, Zhao Ru
Department of Plastic and Reconstructive Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
J Cancer. 2017 Aug 2;8(13):2487-2491. doi: 10.7150/jca.18824. eCollection 2017.
Aberrant expression of microRNAs (miRNAs) are believed to play a central role in the initiation and development of cancer. The aim of our study was to determine the clinical significance of serum miR-10b in melanoma. A total of 85 and 30 serum samples were obtained from patients with melanoma and healthy volunteers respectively. qRT-PCR was performed to evaluate the expression level of miR-10b in the melanoma cell lines and the serum samples from the participants. Then the clinical significance of serum miR-10b was further determined. Our results showed that the expression level of miR-10b was significantly increased in metastasis melanoma cells and melanoma patients compared to their respective controls. In addition, serum miR-10b expression level was able to discriminate melanoma patients from healthy volunteers as well differentiate melanoma patients at different clinical stage with high accuracy. Moreover, upregulation of serum miR-10b was positively associated with enhanced lymph node metastasis, advanced clinical stage and a shortened survival rate. Finally serum miR-10b was an independent prognostic factor for melanoma. Collectively, our study suggests that serum miR-10b level is upregulated in melanoma and associated with poor prognosis. It may be used as a potential prognostic biomarker for melanoma.
微小RNA（miRNA）的异常表达被认为在癌症的发生和发展中起核心作用。我们研究的目的是确定血清miR-10b在黑色素瘤中的临床意义。分别从黑色素瘤患者和健康志愿者中获取了85份和30份血清样本。采用qRT-PCR检测黑色素瘤细胞系及参与者血清样本中miR-10b的表达水平。随后进一步确定血清miR-10b的临床意义。我们的结果显示，与各自的对照相比，转移黑色素瘤细胞和黑色素瘤患者中miR-10b的表达水平显著升高。此外，血清miR-10b表达水平能够准确地区分黑色素瘤患者与健康志愿者，也能区分不同临床分期的黑色素瘤患者。而且，血清miR-10b的上调与淋巴结转移增加、临床分期进展和生存率缩短呈正相关。最后，血清miR-10b是黑色素瘤的一个独立预后因素。总体而言，我们的研究表明黑色素瘤中血清miR-10b水平上调且与预后不良相关。它可能作为黑色素瘤潜在的预后生物标志物。
