Maragal Siddharood, Nagesh G C, Reddy D C Lakshmana, Rao Eguru Sreenivasa
Division of Vegetable Crops, ICAR-Indian Institute of Horticultural Research, Bengaluru, India.
Division of Plant Molecular Biology and Biotechnology, ICAR-Indian Institute of Horticultural Research, Bengaluru, India.
3 Biotech. 2022 Feb;12(2):46. doi: 10.1007/s13205-022-03112-7. Epub 2022 Jan 21.
Rind color and pattern are important external fruit quality traits of watermelon influencing the consumer attention and market acceptance. Varying degrees of green and yellow colors in different combinations forming distinct patterns have been observed on watermelon rind. In the current experiment, an attempt has been made to map the QTLs/genes for four important rind traits, namely, stripe color, stripe pattern, interstripe color, and interstripe pattern. The experiment consisted of two mapping populations namely F (Pop I) and BCF (Pop II) derived from two parental lines, viz., BIL-53 (characterized by medium green marbled rind pattern and yellowish white blotchy interstripe pattern) and IIHR-140-152 (characterized by dark green solid stripes and greenish wavy interstripe pattern). Linkage mapping identified consistent QTLs across populations on chromosome 9. Comparative genomic analysis of these regions revealed two genes, namely, and as potential candidates for stripe and interstripe color. Sequence analysis of gene in parents along with reference genotypes, viz., 97,103 and Charleston gray suggests a 3 bp deletion on 11th exon to be associated with stripe color polymorphism in watermelon.
The online version contains supplementary material available at 10.1007/s13205-022-03112-7.
果皮颜色和花纹是西瓜重要的外观品质性状，影响消费者关注度和市场接受度。在西瓜果皮上已观察到不同程度的绿色和黄色以不同组合形成独特的花纹。在当前实验中，已尝试对四个重要果皮性状的QTL/基因进行定位，这四个性状分别为条纹颜色、条纹图案、条纹间颜色和条纹间图案。该实验由两个作图群体组成，即来自两个亲本系的F（群体I）和BCF（群体II），这两个亲本系分别为BIL-53（特征为中等绿色大理石花纹果皮图案和淡黄白色斑点状条纹间图案）和IIHR-140-152（特征为深绿色实心条纹和绿色波浪状条纹间图案）。连锁图谱绘制在9号染色体上鉴定出了群体间一致的QTL。对这些区域的比较基因组分析揭示了两个基因，即 和 ，作为条纹和条纹间颜色的潜在候选基因。对亲本以及参考基因型97103和查尔斯顿灰中 基因的序列分析表明，第11外显子上的一个3 bp缺失与西瓜条纹颜色多态性相关。
在线版本包含可在10.1007/s13205-022-03112-7获取的补充材料。
