Lee Siyoung, Jang Gaeun, Choi Yunseo, Park Girim, Park Seoyeon, Kwon Gibeom, Je Byoungil, Park Younghoon
Department of Horticultural Bioscience, Pusan National University, Miryang 50463, Korea.
Partner Seeds Co., Ltd., Gimje 54324, Korea.
Plants (Basel). 2022 Oct 17;11(20):2739. doi: 10.3390/plants11202739.
Watermelon fruit rind color (RC) and bloom formation (BF) affect product value and consumer preference. However, information on the candidate gene(s) for additional loci involved in dark green (DG) RC and the genetic control of BF and its major chemical components is lacking. Therefore, this study aimed to identify loci controlling RC and BF using QTL-seq of the F population derived by crossing 'FD061129' with light-green rind and bloom and 'SIT55616RN' with DG rind and bloomless. Phenotypic evaluation of the F and 219 F plants indicated the genetic control of two complementary dominant loci, and , for DG and a dominant locus, , for BF. QTL-seq identified a genomic region on Chr.6 for , Chr.8 for , and Chr.1 for . and helped determine RC with possible environmental effects. Chlorophyll a-b binding protein gene-based CAPS (RC-m5) at matched the highest with the RC phenotype. In the 1.4 cM map interval, two additional gene-based CAPS markers were designed, and the CAPS for a nonsynonymous SNP in , encoding a CSC1-like protein, cosegregated with the BF trait in 219 F plants. Bloom powder showed a high Ca concentration (16,358 mg·kg), indicating that the CSC1-like protein gene is possibly responsible for BF. Our findings provide valuable information for marker-assisted selection for RC and BF and insights into the functional characterization of genes governing these watermelon-fruit-related traits.
西瓜果皮颜色（RC）和蜡粉形成（BF）会影响产品价值和消费者偏好。然而，关于参与深绿色（DG）果皮形成的其他位点的候选基因以及BF及其主要化学成分的遗传控制的信息尚缺。因此，本研究旨在利用‘FD061129’（浅绿色果皮且有蜡粉）与‘SIT55616RN’（深绿色果皮且无蜡粉）杂交产生的F群体的QTL-seq技术来鉴定控制RC和BF的位点。对F和219株F植株的表型评估表明，DG受两个互补显性位点 和 的遗传控制，BF受一个显性位点 的遗传控制。QTL-seq在6号染色体上鉴定出与 相关的基因组区域，在8号染色体上鉴定出与 相关的基因组区域，在1号染色体上鉴定出与 相关的基因组区域。 和 有助于确定可能受环境影响的RC。位于 的基于叶绿素a-b结合蛋白基因的CAPS（RC-m5）与RC表型的匹配度最高。在1.4 cM的 图谱区间内，设计了另外两个基于基因的CAPS标记，位于 中编码类CSC1蛋白的一个非同义SNP的CAPS与219株F植株的BF性状共分离。蜡粉显示出高钙浓度（16358 mg·kg），表明类CSC1蛋白基因可能与BF有关。我们的研究结果为RC和BF的分子标记辅助选择提供了有价值的信息，并为调控这些西瓜果实相关性状的基因的功能表征提供了见解。
