Poindexter N J, Schlievert P M
J Infect Dis. 1986 Apr;153(4):772-9. doi: 10.1093/infdis/153.4.772.
Toxic-shock syndrome toxin-1 (TSST-1), a nonspecific T lymphocyte mitogen, was shown to cause the suppression of immunoglobulin-secreting cells (ISCs) generated in pokeweed mitogen (PWM)-stimulated cultures of human peripheral blood mononuclear cells (PBMs). Greater than 90% suppression of the PWM-stimulated ISC response was achieved when 2 X 10(6) PBMs were cultured in the presence of 1 microgram of TSST-1. The nonspecific suppression induced by the toxin was dose related; amounts of TSST-1 as low as 1.0 ng/2 X 10(6) cultured cells caused significant suppression of the ISC response. TSST-1-induced suppression was due to the activation of a population of cells that secreted a soluble factor that mediated the resulting suppression. PBMs were activated by a 24-hr preincubation of 2 X 10(6) cells in the presence of 1 microgram of TSST-1 followed by repeated washing to remove residual toxin. These cells, when co-cultured with unstimulated PBMs and PWM, suppressed the normal ISC response. The supernatant factor, active at volumes of 10 microliters/2 X 10(6) PBMs, was generated by harvesting the supernatant fluid of TSST-1-activated cells. The results of these in vitro experiments confirm that TSST-1 is an immunosuppressing agent that affects human lymphocyte function and, in such a capacity, may play a role in the development of toxic shock syndrome in humans.
中毒性休克综合征毒素-1（TSST-1）是一种非特异性T淋巴细胞有丝分裂原，已证明它能抑制在美洲商陆有丝分裂原（PWM）刺激的人外周血单个核细胞（PBM）培养物中产生的免疫球蛋白分泌细胞（ISC）。当2×10⁶个PBM在1微克TSST-1存在的情况下培养时，PWM刺激的ISC反应受到大于90%的抑制。毒素诱导的非特异性抑制与剂量相关；低至1.0纳克/2×10⁶个培养细胞的TSST-1量就能显著抑制ISC反应。TSST-1诱导的抑制是由于一群分泌可溶性因子的细胞被激活，该可溶性因子介导了由此产生的抑制作用。通过在1微克TSST-1存在的情况下对2×10⁶个细胞进行24小时预孵育，然后反复洗涤以去除残留毒素，PBM被激活。这些细胞与未刺激的PBM和PWM共培养时，会抑制正常的ISC反应。通过收集TSST-1激活细胞的上清液产生的上清液因子，在10微升/2×10⁶个PBM的体积下具有活性。这些体外实验的结果证实，TSST-1是一种影响人淋巴细胞功能的免疫抑制剂，并且以这种能力可能在人类中毒性休克综合征的发展中起作用。
