Zhao Jiajia, Bao Jinsong, Xu Feifei
Institute of Nuclear Agricultural Sciences, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Bio Protoc. 2022 Jan 20;12(2):e4296. doi: 10.21769/BioProtoc.4296.
The plant nucleus is an important subcellular organelle that contains the genome, ribosomal RNA, and regulatory proteins, and performs a central role in the functioning and metabolism of the cell. Fractionation of intact nuclei is a crucial process to elucidate the function of nuclear proteins. Here, we present a simple method for the fractionation of crude nuclei and extraction of nuclear proteins, based on previously established methods. This protocol provides an easy and quick method to isolate crude nuclei and extract nuclear proteins from seedlings, which is useful for the research on the nuclear proteins, without requirement for high-purity nuclei. Graphic abstract: Schematic procedure for the isolation of crude nuclei and extraction of nuclear proteins from seedlings.
植物细胞核是一种重要的亚细胞器，它包含基因组、核糖体RNA和调节蛋白，并在细胞的功能和代谢中发挥核心作用。完整细胞核的分级分离是阐明核蛋白功能的关键过程。在此，我们基于先前建立的方法，提出一种简单的粗细胞核分级分离和核蛋白提取方法。该方案提供了一种从幼苗中分离粗细胞核和提取核蛋白的简便快速方法，这对于核蛋白的研究很有用，且不需要高纯度的细胞核。图形摘要：从幼苗中分离粗细胞核和提取核蛋白的示意图。
