Ji You-Ren, Hsu Ya-Hsiang, Syue Ming-Hua, Wang Ying-Chu, Lin Shyr-Yi, Huang Tsung-Wei, Young Tai-Horng
Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan.
Division of Gastroenterology, Department of Internal Medicine, Wan Fang Hospital, Taipei Medical University, Taipei 116, Taiwan.
ACS Omega. 2022 Jan 21;7(4):3254-3261. doi: 10.1021/acsomega.1c05178. eCollection 2022 Feb 1.
In this study, superabsorbent polyelectrolyte hydrogels were synthesized by cross-linking a nondegradable poly (allylamine hydrochloride) (PAH) and a recombinant protein with a specific enzymatic cleavage site. The recombinant protein was produced by with the pET-32b(+) plasmid, which is featured with the thioredoxin (Trx) gene containing a thrombin recognition site and a T7/lac hybrid promoter for high expression of recombinant protein. The swelling test shows that the composite hydrogel still maintained a high swelling ratio to 900% when 15% recombinant protein was cross-linked with PAH. The degradation test shows that such a PAH composite hydrogel could be decomposed by the addition of specific enzyme thrombin, which might lead to new biomedical applications of hydrogels needed to be decomposable by specific time not determined by the time period.
在本研究中，通过将不可降解的聚（烯丙胺盐酸盐）（PAH）与具有特定酶切位点的重组蛋白交联，合成了超吸水性聚电解质水凝胶。重组蛋白由pET-32b(+)质粒产生，该质粒的特征是硫氧还蛋白（Trx）基因含有凝血酶识别位点和T7/lac杂交启动子，用于重组蛋白的高表达。溶胀试验表明，当15%的重组蛋白与PAH交联时，复合水凝胶仍保持高达900%的高溶胀率。降解试验表明，这种PAH复合水凝胶可通过添加特定的凝血酶进行分解，这可能会带来水凝胶新的生物医学应用，即需要在特定时间（而非由时间段决定）进行分解。
