Ashkenazi S
J Med Microbiol. 1986 Mar;21(2):117-23. doi: 10.1099/00222615-21-2-117.
The adherence properties of non-fimbriate entero-invasive Escherichia coli O124 in the guinea pig intestinal tract were studied. Quantitative in-vitro determinations were done by incubating radiolabelled bacteria with suspensions of viable intestinal cells released by treating loops of the guinea pig intestine with solutions containing EDTA, dithiothreitol and citrate. Non-bound bacteria were separated from the intestinal cells on a Percoll gradient. Only cells released from the colon, especially from its transverse and descending regions, avidly adhered to E. coli O124 (68-79 bacteria/cell), whereas the attachment to ileal cells was negligible. The adherence process was Ca++ and temperature-dependent, had an optimal pH of 6.2 and was inhibited by fucose, glucose or mannose. Several pretreatment studies of the bacteria or the colonic cells showed that the adherence was mediated by a carbohydrate-binding protein (adhesin or lectin) on the colonic cells and not on the bacterial surface. Results of studies of in-vitro adherence to intestinal loops and to intact intestinal surfaces correlated well with the in-vitro findings. These results indicate that the adherence of entero-invasive E. coli O124 to the gut is similar to the attachment of Shigella flexneri and is quite different from that of enterotoxigenic E. coli.
对非纤毛肠侵袭性大肠杆菌O124在豚鼠肠道中的黏附特性进行了研究。通过将放射性标记的细菌与用含有乙二胺四乙酸、二硫苏糖醇和柠檬酸盐的溶液处理豚鼠肠袢释放的活肠细胞悬液孵育，进行了定量体外测定。未结合的细菌通过Percoll梯度从肠细胞中分离出来。只有从结肠，特别是其横部和降部释放的细胞，能强烈黏附于大肠杆菌O124（68 - 79个细菌/细胞），而对回肠细胞的黏附则可忽略不计。黏附过程依赖于Ca++和温度，最适pH为6.2，并受到岩藻糖、葡萄糖或甘露糖的抑制。对细菌或结肠细胞的多项预处理研究表明黏附是由结肠细胞而非细菌表面的一种碳水化合物结合蛋白（黏附素或凝集素）介导的。对肠袢和完整肠表面的体外黏附研究结果与体外研究结果相关性良好。这些结果表明肠侵袭性大肠杆菌O124对肠道的黏附类似于弗氏志贺菌的黏附，与产肠毒素大肠杆菌的黏附截然不同。
