Department of Biotechnology, Dalian Medical University, Dalian 116044, China.
Biomed Pharmacother. 2017 Oct;94:468-473. doi: 10.1016/j.biopha.2017.07.123. Epub 2017 Aug 2.
Our research group previously isolated and identified a strain of pathogenic Escherichia coli from clinical samples called E. coli O124 K72. The present study was aimed at determining the potential effects of E. coli O124 K72 on intestinal barrier functions and structural proteins integrity in guinea pig. Guinea pigs were grouped into three groups; control (CG); E. coli O124 K72 (E. coli); and probiotics Lactobacillus rhamnosus (LGG). Initially, we create intestinal dysbiosis by giving all animals Levofloxacin for 10days, but the control group (CG) received the same volume of saline. Then, the animals received either E. coli O124 K72 (E. coli) or Lactobacillus rhamnosus (LGG) according to their assigned group. E. coli O124 K72 treatment significantly affected colon morphology and distorted intestinal barrier function by up-regulating Claudin2 and down-regulating Occludin. In addition, E. coli upregulated the mRNA expression of MUC1, MUC2, MUC13 and MUC15. Furthermore, suspected tumor was found in the E. coli treated animals. Our results suggested that E. coli O124 K72 strain has adverse effects on intestinal barrier functions and is capable of altering integrity of structural proteins in guinea pig model while at same time it may have a role in colon carcinogenesis.
我们的研究小组之前从临床样本中分离并鉴定出一种致病性大肠杆菌菌株，称为大肠杆菌 O124 K72。本研究旨在确定大肠杆菌 O124 K72 对豚鼠肠道屏障功能和结构蛋白完整性的潜在影响。豚鼠分为三组：对照组（CG）；大肠杆菌 O124 K72（大肠杆菌）；和益生菌鼠李糖乳杆菌（LGG）。最初，我们通过给所有动物左氧氟沙星 10 天来建立肠道菌群失调，但对照组（CG）接受相同体积的生理盐水。然后，根据分配的组别，动物分别接受大肠杆菌 O124 K72（大肠杆菌）或鼠李糖乳杆菌（LGG）。大肠杆菌 O124 K72 处理显著影响结肠形态，并通过上调 Claudin2 和下调 Occludin 来破坏肠道屏障功能。此外，大肠杆菌上调 MUC1、MUC2、MUC13 和 MUC15 的 mRNA 表达。此外，在大肠杆菌处理的动物中发现了疑似肿瘤。我们的结果表明，大肠杆菌 O124 K72 菌株对肠道屏障功能有不良影响，能够改变豚鼠模型中结构蛋白的完整性，同时可能在结肠癌变中起作用。
