Zhu Xia, Chen Yiyi, Tang Xinyao, Wang Dongxia, Miao Yeqiu, Zhang Jing, Li Ruirui, Zhang Lishi, Chen Jinyao
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China.
Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu, China.
J Appl Toxicol. 2022 Aug;42(8):1310-1322. doi: 10.1002/jat.4297. Epub 2022 Feb 13.
The mycotoxin altertoxin I (ATX-I) is one of secondary metabolites produced by Alternaria fungi and is frequently detected as food and feed contaminants. Little is known about the genotoxicity of the ATX-I. In order to evaluate potential genotoxicity and general toxicity of ATX-I, the novel 28-day multiendpoint (Pig-a assay + micronucleus [MN] test + comet assay) genotoxicity platform was applied. Male Sprague-Dawley (SD) rats were randomized to five groups (six rats per group), that is, a positive control group (N-ethyl-N-nitrosourea [ENU], 40 mg/kg.bw/d), two solvent control groups (PBS and corn oil), and two ATX-I-treated groups (low-dose group [1.10 μg/kg.bw/d] and high-dose group [5.51 μg/kg.bw/d]). Treatments were administered by oral gavage to male SD rats for 28 consecutive days. Histopathological damages in the liver, kidney, and spleen were observed, but without significant changes in hematological and serum biochemical parameters. Genotoxic endpoints indicated that ATX-I could cause DNA damage. To summarize, in a relatively low-dose range, ATX-I may not have direct genotoxicity in vivo but could induce liver, kidney, and spleen damage.
霉菌毒素互隔交链孢酚单甲醚（ATX-I）是链格孢属真菌产生的次生代谢产物之一，常被检测出是食品和饲料中的污染物。关于ATX-I的遗传毒性知之甚少。为了评估ATX-I的潜在遗传毒性和一般毒性，应用了新型的28天多终点（猪-a试验+微核[MN]试验+彗星试验）遗传毒性平台。将雄性Sprague-Dawley（SD）大鼠随机分为五组（每组六只大鼠），即阳性对照组（N-乙基-N-亚硝基脲[ENU]，40mg/kg体重/天）、两个溶剂对照组（PBS和玉米油）以及两个ATX-I处理组（低剂量组[1.10μg/kg体重/天]和高剂量组[5.51μg/kg体重/天]）。连续28天经口灌胃给予雄性SD大鼠进行处理。观察到肝脏、肾脏和脾脏的组织病理学损伤，但血液学和血清生化参数无显著变化。遗传毒性终点表明ATX-I可导致DNA损伤。总之，在相对低剂量范围内，ATX-I在体内可能没有直接遗传毒性，但可诱导肝脏、肾脏和脾脏损伤。
