Division of Veterinary Biotechnology, ICAR-IVRI, Bareilly, India.
Department of Biological Sciences, SHUATS, Allahabad, India.
Virulence. 2022 Dec;13(1):310-322. doi: 10.1080/21505594.2022.2026564.
(PPR) characterized by fever, sore mouth, conjunctivitis, gastroenteritis, and pneumonia, is an acute, highly contagious viral disease of sheep and goats. The role of long non-coding RNAs (lncRNAs) in PPRV infection has not been explored to date. In this study, the transcriptome profiles of virulent Peste des petits ruminants virus (PPRV) infected goat tissues - lung and spleen were analyzed to identify the role of lncRNAs in PPRV infection. A total of 13,928 lncRNA transcripts were identified, out of which 170 were known lncRNAs. Intergenic lncRNAs (7625) formed the major chunk of the novel lncRNA transcripts. Differential expression analysis revealed that 15 lncRNAs (11 downregulated and 4 upregulated) in the PPRV infected spleen samples and 16 lncRNAs (13 downregulated and 3 upregulated) in PPRV infected lung samples were differentially expressed as compared to control. The differentially expressed lncRNAs (DElncRNAs) possibly regulate various immunological processes related to natural killer cell activation, antigen processing and presentation, and B cell activity, by regulating the expression of mRNAs through the cis- or trans-regulatory mechanism. Functional enrichment analysis of differentially expressed mRNAs (DEmRNAs) revealed enrichment of immune pathways and biological processes in concordance with the pathways in which correlated lncRNA-neighboring genes were enriched. The results suggest that a coordinated immune response is raised in both lung and spleen tissues of the goat through mRNA-lncRNA crosstalk.
（PPR）以发热、口疮、结膜炎、胃肠炎和肺炎为特征，是一种急性、高度传染性的绵羊和山羊病毒病。长链非编码 RNA（lncRNA）在 PPRV 感染中的作用迄今尚未得到探索。在这项研究中，分析了强毒小反刍兽疫病毒（PPRV）感染山羊组织-肺和脾的转录组谱，以确定 lncRNA 在 PPRV 感染中的作用。共鉴定出 13928 个 lncRNA 转录本，其中 170 个是已知的 lncRNA。基因间 lncRNA（7625）构成了新的 lncRNA 转录本的主要部分。差异表达分析显示，与对照相比，PPRV 感染的脾脏样本中有 15 个 lncRNA（11 个下调和 4 个上调），PPRV 感染的肺样本中有 16 个 lncRNA（13 个下调和 3 个上调）差异表达。差异表达的 lncRNA（DElncRNA）可能通过顺式或反式调控机制调节 mRNAs 的表达，从而调节与自然杀伤细胞激活、抗原加工和呈递以及 B 细胞活性相关的各种免疫过程。差异表达的 mRNAs（DEmRNAs）的功能富集分析显示，与相关 lncRNA 邻近基因富集的途径一致，免疫途径和生物学过程富集。结果表明，通过 mRNA-lncRNA 串扰，在山羊的肺和脾组织中引发了协调一致的免疫反应。
