Wang Haixia, Huang Hong, Jiang Ning, Zhang Yiwen, Lv Jingwei, Liu Xinmin
The National Clinical Research Center for Mental Disorders & Beijing Key Laboratory of Mental Disorders, Beijing Anding Hospital, Capital Medical University, Beijing, China.
Research Center of Pharmacology and Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Phytother Res. 2022 Mar;36(3):1402-1412. doi: 10.1002/ptr.7402. Epub 2022 Feb 7.
The general consensus is that stress affects the central nervous system and can lead to cognitive problems. The root of Polygala tenuifolia (P. tenuifolia) is a well-known traditional Chinese medicine used for improving brain function. Tenuifolin (TEN) is the major constituent of P. tenuifolia and has a promising neuroprotective property. The purpose of this study was to investigate the alleviating effect of TEN on cognitive impairment induced by chronic restraint stress (CRS) and its mechanism. Our results showed that CRS exposure resulted in impaired cognitive performance in C57BL/6J mice, as indicated by decreased responses in Y-maze, novel objects recognition, and step-through passive avoidance tests. TEN treated daily orally (10 and 20 mg/kg) for 30 days reversed these behavior changes. Meanwhile, TEN could significantly regulate interleukin (IL)-6 and IL-10 levels in the hippocampus. TEN inhibited the toll-like receptor 4/nuclear factor-kappa B-mediated inflammation, as well as adrenocorticotropic hormone and corticosterone levels in serum. Most importantly, we found that TEN also upregulated the expressions of brain-derived neurotrophic factor, tropomyosin kinase B, glucocorticoid receptor, glutamate receptor 1, and synapse-associated proteins. Collectively, these data suggest that TEN has a potential improvement effect on memory loss caused by CRS.
普遍的共识是，压力会影响中枢神经系统并可能导致认知问题。远志是一种著名的用于改善脑功能的传统中药。细叶远志皂苷是远志的主要成分，具有良好的神经保护特性。本研究的目的是探讨细叶远志皂苷对慢性束缚应激（CRS）诱导的认知障碍的缓解作用及其机制。我们的结果表明，暴露于CRS会导致C57BL/6J小鼠的认知能力受损，如Y迷宫、新物体识别和穿梭式被动回避试验中的反应降低所示。每天口服细叶远志皂苷（10和20毫克/千克），持续30天，可逆转这些行为变化。同时，细叶远志皂苷可显著调节海马中的白细胞介素（IL）-6和IL-10水平。细叶远志皂苷抑制Toll样受体4/核因子-κB介导的炎症以及血清中的促肾上腺皮质激素和皮质酮水平。最重要的是，我们发现细叶远志皂苷还上调了脑源性神经营养因子、原肌球蛋白激酶B、糖皮质激素受体、谷氨酸受体1和突触相关蛋白的表达。总体而言，这些数据表明细叶远志皂苷对CRS引起的记忆丧失具有潜在的改善作用。
