• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

编码酵母细胞质天冬氨酰 - tRNA合成酶（APS）的基因的核苷酸序列；天冬氨酰 - tRNA合成酶（AspRS）mRNA 5'和3'末端的定位

Nucleotide sequence of the gene coding for yeast cytoplasmic aspartyl-tRNA synthetase (APS); mapping of the 5' and 3' termini of AspRS mRNA.

作者信息

Sellami M, Fasiolo F, Dirheimer G, Ebel J P, Gangloff J

出版信息

Nucleic Acids Res. 1986 Feb 25;14(4):1657-66. doi: 10.1093/nar/14.4.1657.

DOI:10.1093/nar/14.4.1657
PMID:3513127
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339550/
Abstract

A 3.8 Kb DNA fragment, which contains the structural gene of aspartyl-tRNA synthetase (AspRS) and its flanking regions, has been fully sequenced by the combined M13/dideoxy chain terminator method. From the single open reading frame of correct length (1671 bp) we deduced an amino acid sequence consistent with that of several peptides of AspRS. No significant internal sequence repeats were observed in the primary structure of the protein. The AspRS gene (APS) has a codon usage pattern typical of non abundant proteins. S1 nuclease analysis of APS mRNA showed a major start 17 bases downstream from a "TATA box" and stops near an RNA polymerase terminator sequence.

摘要

一个包含天冬氨酰 - tRNA合成酶（AspRS）结构基因及其侧翼区域的3.8 Kb DNA片段，已通过M13/双脱氧链终止法组合进行了全序列测定。从正确长度（1671 bp）的单一开放阅读框中，我们推导了与AspRS的几个肽段序列一致的氨基酸序列。在该蛋白质的一级结构中未观察到明显的内部序列重复。AspRS基因（APS）具有非丰富蛋白质典型的密码子使用模式。对APS mRNA的S1核酸酶分析表明，主要起始位点在一个“TATA框”下游17个碱基处，终止于一个RNA聚合酶终止序列附近。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7116/339550/79e432b85b2f/nar00273-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7116/339550/79e432b85b2f/nar00273-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7116/339550/79e432b85b2f/nar00273-0126-a.jpg

相似文献

1
Nucleotide sequence of the gene coding for yeast cytoplasmic aspartyl-tRNA synthetase (APS); mapping of the 5' and 3' termini of AspRS mRNA.编码酵母细胞质天冬氨酰 - tRNA合成酶（APS）的基因的核苷酸序列；天冬氨酰 - tRNA合成酶（AspRS）mRNA 5'和3'末端的定位
Nucleic Acids Res. 1986 Feb 25;14(4):1657-66. doi: 10.1093/nar/14.4.1657.
2
Cloning and characterization of the gene coding for cytoplasmic seryl-tRNA synthetase from Saccharomyces cerevisiae.酿酒酵母细胞质丝氨酰 - tRNA合成酶编码基因的克隆与鉴定
Nucleic Acids Res. 1987 Mar 11;15(5):1887-904. doi: 10.1093/nar/15.5.1887.
3
Isolation and characterization of the yeast aspartyl-tRNA synthetase gene.酵母天冬氨酰 - tRNA合成酶基因的分离与鉴定
Gene. 1985;40(2-3):349-52. doi: 10.1016/0378-1119(85)90060-5.
4
Homology of aspartyl- and lysyl-tRNA synthetases.天冬氨酰-tRNA合成酶和赖氨酰-tRNA合成酶的同源性。
Proc Natl Acad Sci U S A. 1989 Aug;86(16):6023-7. doi: 10.1073/pnas.86.16.6023.
5
Aspartyl-tRNA synthetase from Escherichia coli: cloning and characterisation of the gene, homologies of its translated amino acid sequence with asparaginyl- and lysyl-tRNA synthetases.来自大肠杆菌的天冬氨酰 - tRNA合成酶：基因的克隆与特性分析，其翻译后的氨基酸序列与天冬酰胺酰 - tRNA合成酶和赖氨酰 - tRNA合成酶的同源性
Nucleic Acids Res. 1990 Dec 11;18(23):7109-18. doi: 10.1093/nar/18.23.7109.
6
An intricate RNA structure with two tRNA-derived motifs directs complex formation between yeast aspartyl-tRNA synthetase and its mRNA.一种具有两个源自tRNA的基序的复杂RNA结构指导酵母天冬氨酰-tRNA合成酶与其mRNA之间形成复合物。
J Mol Biol. 2005 Dec 2;354(3):614-29. doi: 10.1016/j.jmb.2005.09.063. Epub 2005 Oct 5.
7
Asparaginyl-tRNA synthetase from Escherichia coli has significant sequence homologies with yeast aspartyl-tRNA synthetase.来自大肠杆菌的天冬酰胺酰-tRNA合成酶与酵母天冬氨酸-tRNA合成酶具有显著的序列同源性。
Gene. 1989 Dec 14;84(2):481-5. doi: 10.1016/0378-1119(89)90524-6.
8
Study of the arrangement of the functional domains along the yeast cytoplasmic aspartyl-tRNA synthetase.酵母细胞质天冬氨酰 - tRNA合成酶功能结构域排列的研究。
Eur J Biochem. 1989 Mar 15;180(2):351-8. doi: 10.1111/j.1432-1033.1989.tb14655.x.
9
Sequence polymorphisms in the yeast gene encoding aspartyl tRNA synthase.编码天冬氨酰tRNA合成酶的酵母基因中的序列多态性。
Nucleic Acids Res. 1988 Feb 11;16(3):1212. doi: 10.1093/nar/16.3.1212.
10
cDNA sequence, predicted primary structure, and evolving amphiphilic helix of human aspartyl-tRNA synthetase.人天冬氨酰 - tRNA合成酶的cDNA序列、预测的一级结构及进化的两亲性螺旋
J Biol Chem. 1989 Oct 5;264(28):16608-12.

引用本文的文献

1
Plasmodial aspartyl-tRNA synthetases and peculiarities in Plasmodium falciparum.疟原虫天冬氨酰 - tRNA合成酶与恶性疟原虫的特性
J Biol Chem. 2009 Jul 10;284(28):18893-903. doi: 10.1074/jbc.M109.015297. Epub 2009 May 14.
2
A domain in the N-terminal extension of class IIb eukaryotic aminoacyl-tRNA synthetases is important for tRNA binding.IIb类真核生物氨酰-tRNA合成酶N端延伸区中的一个结构域对tRNA结合很重要。
EMBO J. 2000 May 15;19(10):2371-80. doi: 10.1093/emboj/19.10.2371.
3
Molecular cloning, sequence, structural analysis and expression of the histidyl-tRNA synthetase gene from Streptococcus equisimilis.

本文引用的文献

1
Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.侧翼核苷酸在真核生物核糖体识别AUG起始密码子中的可能作用。
Nucleic Acids Res. 1981 Oct 24;9(20):5233-52. doi: 10.1093/nar/9.20.5233.
2
Codon selection in yeast.酵母中的密码子选择
J Biol Chem. 1982 Mar 25;257(6):3026-31.
3
Crystallographic studies on the aspartyl-tRNA synthetase-tRNAAsp system from yeast. The crystalline aminoacyl-tRNA synthetase.酵母中天冬氨酰 - tRNA合成酶 - tRNAAsp系统的晶体学研究。结晶的氨酰 - tRNA合成酶。
马链球菌组马疫链球菌组氨酸-tRNA合成酶基因的分子克隆、序列、结构分析及表达
Nucleic Acids Res. 1993 Feb 11;21(3):615-20. doi: 10.1093/nar/21.3.615.
4
Role of dimerization in yeast aspartyl-tRNA synthetase and importance of the class II invariant proline.二聚化在酵母天冬氨酰 - tRNA合成酶中的作用以及II类不变脯氨酸的重要性。
Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10816-20. doi: 10.1073/pnas.90.22.10816.
5
The class II aminoacyl-tRNA synthetases and their active site: evolutionary conservation of an ATP binding site.II类氨酰-tRNA合成酶及其活性位点：ATP结合位点的进化保守性
J Mol Evol. 1995 May;40(5):499-508. doi: 10.1007/BF00166618.
6
Sequence polymorphisms in the yeast gene encoding aspartyl tRNA synthase.编码天冬氨酰tRNA合成酶的酵母基因中的序列多态性。
Nucleic Acids Res. 1988 Feb 11;16(3):1212. doi: 10.1093/nar/16.3.1212.
7
Cloning and characterization of the gene coding for cytoplasmic seryl-tRNA synthetase from Saccharomyces cerevisiae.酿酒酵母细胞质丝氨酰 - tRNA合成酶编码基因的克隆与鉴定
Nucleic Acids Res. 1987 Mar 11;15(5):1887-904. doi: 10.1093/nar/15.5.1887.
8
Homology of aspartyl- and lysyl-tRNA synthetases.天冬氨酰-tRNA合成酶和赖氨酰-tRNA合成酶的同源性。
Proc Natl Acad Sci U S A. 1989 Aug;86(16):6023-7. doi: 10.1073/pnas.86.16.6023.
9
Homology of lysS and lysU, the two Escherichia coli genes encoding distinct lysyl-tRNA synthetase species.lysS和lysU的同源性，这两个大肠杆菌基因编码不同种类的赖氨酰-tRNA合成酶。
Nucleic Acids Res. 1990 Jan 25;18(2):305-12. doi: 10.1093/nar/18.2.305.
10
Aspartyl-tRNA synthetase from Escherichia coli: cloning and characterisation of the gene, homologies of its translated amino acid sequence with asparaginyl- and lysyl-tRNA synthetases.来自大肠杆菌的天冬氨酰 - tRNA合成酶：基因的克隆与特性分析，其翻译后的氨基酸序列与天冬酰胺酰 - tRNA合成酶和赖氨酰 - tRNA合成酶的同源性
Nucleic Acids Res. 1990 Dec 11;18(23):7109-18. doi: 10.1093/nar/18.23.7109.
J Mol Biol. 1980 Mar 25;138(1):129-35. doi: 10.1016/s0022-2836(80)80008-8.
4
Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.通过对体内特定缺失突变体的研究鉴定H2A组蛋白基因前奏序列中的调控序列。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1432-6. doi: 10.1073/pnas.77.3.1432.
5
Primary structure of aspartyl-tRNA synthetase from baker's yeast: tryptic and CNBr peptides.
Biochimie. 1983 Jul;65(7):379-88. doi: 10.1016/s0300-9084(83)80057-1.
6
Large scale purification and structural properties of yeast aspartyl-tRNA synthetase.酵母天冬氨酰 - tRNA合成酶的大规模纯化及结构特性
Biochem Biophys Res Commun. 1983 Nov 30;117(1):259-67. doi: 10.1016/0006-291x(83)91569-3.
7
Transcription terminates in yeast distal to a control sequence.转录在酵母中于一个控制序列远端终止。
Cell. 1983 Jun;33(2):607-14. doi: 10.1016/0092-8674(83)90441-5.
8
Repeated DNA sequences upstream from HIS1 also occur at several other co-regulated genes in Saccharomyces cerevisiae.HIS1上游的重复DNA序列也出现在酿酒酵母中其他几个共同调控的基因处。
J Biol Chem. 1983 Apr 25;258(8):5238-47.
9
Nucleotide sequence of yeast LEU2 shows 5'-noncoding region has sequences cognate to leucine.酵母LEU2的核苷酸序列表明，5'-非编码区具有与亮氨酸同源的序列。
Cell. 1982 Dec;31(2 Pt 1):319-25. doi: 10.1016/0092-8674(82)90125-8.
10
Structure of the Schizosaccharomyces pombe cytochrome c gene.粟酒裂殖酵母细胞色素c基因的结构
Mol Cell Biol. 1982 Feb;2(2):106-16. doi: 10.1128/mcb.2.2.106-116.1982.