对从南美最大都市地区分离出的耐环丙沙星淋病奈瑟菌菌株进行的基因组分析。

Genomic analyses of ciprofloxacin-resistant Neisseria gonorrhoeae isolates recovered from the largest South American metropolitan area.

作者信息

Cassu-Corsi Dandara, Santos Fernanda F, Cayô Rodrigo, Martins Willames M B S, Nodari Carolina S, Almeida Luiz G P, Martins Rafael A, Carvalho da Silva Roberto J, Vasconcelos Ana Tereza R, Pignatari Antônio C C, Gales Ana C

机构信息

Universidade Federal de São Paulo (UNIFESP), Laboratório ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil; Programa de Pós Graduação em Medicina Translacional, Departamento de Medicina, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil.

Universidade Federal de São Paulo (UNIFESP), Laboratório ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil.

出版信息

Genomics. 2022 Mar;114(2):110287. doi: 10.1016/j.ygeno.2022.110287. Epub 2022 Feb 4.

DOI:10.1016/j.ygeno.2022.110287

PMID:35131478

Abstract

We sequenced 13 Neisseria gonorrhoeae isolates exhibiting distinct susceptibility profiles and which were recovered over 12 years in the metropolitan region of São Paulo, Brazil. Whole Genome Sequencing (WGS) was performed on an Illumina MiSeq™ 2 × 300 bp paired-end reads. Bioinformatics analyses were carried out using CGE, PATRIC, and BLAST databases for manual curation of obtained genomes. Multilocus sequence typing (MLST) analysis identified seven STs, namely ST1580, ST1590, ST1901, ST1902, ST8161, ST9363, and ST15640. Moreover, a diversity of mutations was observed in MtrR/G45D-A39T, PIB/G120K-A121S, and PBP1/L421P. Mutations associated with sulfonamides (DHPS/R228S) and rifampicin (RNAP/H552N) were also detected, as well as tetracycline resistance determinants, namely rpsJ/V57M and tet(M). The results presented herein can contribute to the knowledge of N. gonorrhoeae strains circulating in Sao Paulo, Brazil.

摘要

我们对13株淋病奈瑟菌分离株进行了测序，这些分离株表现出不同的药敏谱，是在巴西圣保罗大都市区12年期间分离得到的。使用Illumina MiSeq™ 进行全基因组测序，生成2×300 bp双端 reads。利用CGE、PATRIC和BLAST数据库进行生物信息学分析，以手动筛选获得的基因组。多位点序列分型（MLST）分析确定了7个序列型，即ST1580、ST1590、ST1901、ST1902、ST8161、ST9363和ST15640。此外，在MtrR/G45D-A39T、PIB/G120K-A121S和PBP1/L421P中观察到多种突变。还检测到与磺胺类药物（DHPS/R228S）和利福平（RNAP/H552N）相关的突变，以及四环素耐药决定簇，即rpsJ/V57M和tet(M)。本文给出的结果有助于了解巴西圣保罗市流行的淋病奈瑟菌菌株。