Departamento de Reprodução e Avaliação Animal, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, 23897-000, Brazil; Eutheria Foundation, Cross Plains, WI, 53528, USA; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Departamento de Reprodução e Avaliação Animal, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, 23897-000, Brazil.
Theriogenology. 2022 Apr 1;182:78-84. doi: 10.1016/j.theriogenology.2022.01.032. Epub 2022 Jan 29.
Prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) are involved in equine embryo mobility throughout the uterus on Days 11-15 (ovulation = Day 0). On a day (Day 12) of maximal embryo mobility in pregnant mares (n = 13) and before luteolysis in nonbred mares (n = 10), gene expressions were compared between the uterine horns that did and did not contain the mobile embryo and between pregnant and nonbred mares. A cytobrush was used to collect an endometrial sample from the middle of each uterine horn. In nonbred mares, there was no difference for any of the considered gene expressions between the uterine horn ipsilateral and contralateral to the CL or for side (left vs right). For endometrial estrogen receptors, ESR1 was lower (P < 0.03) and ESR2 was greater (P < 0.04) for pregnant than nonbred mares. The mRNA abundance for PGE2 synthase (PTGES) was greater (P < 0.05) in the horn with (1.40 ± 0.10) than without (0.89 ± 0.10) the embryo and was greater (P < 0.05) in the horn with the embryo than in the combined horns of nonbred mares (1.06 ± 0.10). The hypothesis that the embryo locally upregulates PGE2 and PGF2α synthesis in the endometrium adjacent to the embryo in the pregnant group but not in the uterine horns of the nonbred group, was partially supported; only PGE2 synthase (PTGES) was locally upregulated in the endometrium adjacent to the mobile embryo.
前列腺素 E2（PGE2）和前列腺素 F2α（PGF2α）参与马胚胎在排卵后第 11-15 天（排卵=第 0 天）在子宫内的移动。在怀孕母马胚胎移动性最大的一天（第 12 天，n=13）和未发情母马黄体溶解之前（n=10），比较了含有和不含有移动胚胎的子宫角之间以及怀孕和未发情母马之间的基因表达。使用细胞刷从中部收集每个子宫角的子宫内膜样本。在未发情母马中，同侧和对侧 CL 的子宫角之间或同侧（左 vs 右）的任何基因表达都没有差异。对于子宫内膜雌激素受体，ESR1 低于（P<0.03），ESR2 高于（P<0.04）怀孕母马。PTGES 的 mRNA 丰度在有胚胎的子宫角中更高（P<0.05）（1.40±0.10），而在没有胚胎的子宫角中更低（0.89±0.10），并且在有胚胎的子宫角中高于未发情母马的联合子宫角（1.06±0.10）。胚胎局部上调妊娠组子宫内膜中紧邻胚胎的 PGE2 和 PGF2α 合成，而不是未发情组子宫角中 PGE2 和 PGF2α 合成的假设得到部分支持；仅 PGE2 合酶（PTGES）在紧邻移动胚胎的子宫内膜中局部上调。
