Piotrowska-Tomala Katarzyna K, Szóstek-Mioduchowska Anna Z, Drzewiecka Ewa M, Jonczyk Agnieszka W, Wójtowicz Anna, Wrobel Michał H, Ferreira-Dias Graca, Skarzynski Dariusz J
Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research Polish Academy of Science, Olsztyn, Poland.
Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research Polish Academy of Science, Olsztyn, Poland.
Front Vet Sci. 2024 Dec 5;11:1479508. doi: 10.3389/fvets.2024.1479508. eCollection 2024.
Prostaglandins (PG) are important regulators of the myometrial contractility in mammals. Endometrosis, a condition characterized by morphological changes in the equine endometrium, also affects endometrial secretory function. However, it remains unclear whether and how endometrosis affects myometrial function.
This study investigated: (i) mRNA transcription of genes encoding specific enzymes responsible for PG synthesis, such as prostaglandin-endoperoxide synthase (), PGE synthase (), PGF synthase () and : PGE receptors (), and PGF receptor () in equine myometrium and, (ii) the effects of PGE and PGF on myometrial contractile activity, during endometrosis in mares. The myometria used in experiments 1 and 2 were collected from mares in the mid-luteal ( = 23) and follicular ( = 20) phases of the estrous cycle, according to the histological classification of the endometrium (Kenney and Doig categories I, IIA, IIB, and III).
In experiment 1, changes in mRNA transcription of or in the myometrium during the course of endometrosis were determined using qPCR. During the mid-luteal phase, myometrial mRNA transcription of increased in mares with endometrial category IIB compared to category I. However, myometrial mRNA transcription of decreased during the progression of endometrosis compared to category I. During the follicular phase, mRNA transcription of and increased in mares with endometrial categories III or IIA, respectively. In addition, mRNA transcription of increased in mares with endometrium category IIA compared to category I. In experiment 2, the force of myometrial contractions was measured using an isometric concentration transducer. In the follicular phase, PGE decreased the force of contractions in mares with endometrial categories IIA, IIB, and III compared to the respective control groups. Prostaglandin F increased the force of myometrial contractions in mares with category IIA endometrium, whereas it decreased in category IIB compared to the respective control groups.
We concluded that in the progression of endometrosis there are changes in the myometrial transcription of mRNA encoding and , particularly and . Mares with endometrosis had abnormal myometrial contractile responses to PG. These findings suggest that myometrial function may be compromised during the progression of endometrosis.
前列腺素（PG）是哺乳动物子宫肌层收缩性的重要调节因子。子宫内膜异位症是一种以马子宫内膜形态变化为特征的病症，也会影响子宫内膜的分泌功能。然而，子宫内膜异位症是否以及如何影响子宫肌层功能仍不清楚。
本研究调查了：（i）编码负责PG合成的特定酶的基因的mRNA转录，如前列腺素内过氧化物合酶（）、PGE合酶（）、PGF合酶（）以及：PGE受体（）和PGF受体（）在马子宫肌层中的情况，以及（ii）在母马子宫内膜异位症期间PGE和PGF对子宫肌层收缩活性的影响。实验1和2中使用的子宫肌层是根据子宫内膜的组织学分类（肯尼和多伊格分类I、IIA、IIB和III）从发情周期黄体中期（ = 23）和卵泡期（ = 20）的母马中收集的。
在实验1中，使用qPCR测定子宫内膜异位症过程中子宫肌层中或的mRNA转录变化。在黄体中期，与I类相比，IIB类子宫内膜母马的子宫肌层mRNA转录增加。然而，与I类相比，在子宫内膜异位症进展过程中子宫肌层的mRNA转录减少。在卵泡期，III类或IIA类子宫内膜母马的和的mRNA转录分别增加。此外，与I类相比，IIA类子宫内膜母马的mRNA转录增加。在实验2中，使用等长浓度换能器测量子宫肌层收缩力。在卵泡期，与各自的对照组相比，PGE降低了IIA、IIB和III类子宫内膜母马的收缩力。前列腺素F增加了IIA类子宫内膜母马的子宫肌层收缩力，而与各自的对照组相比，IIB类则降低。
我们得出结论，在子宫内膜异位症的进展过程中，编码和的mRNA的子宫肌层转录存在变化，特别是和。患有子宫内膜异位症的母马对PG的子宫肌层收缩反应异常。这些发现表明，在子宫内膜异位症进展过程中子宫肌层功能可能受到损害。
