酵母和哺乳动物利用相似的胞质成分来驱动蛋白质通过高尔基体复合体进行转运。
Yeast and mammals utilize similar cytosolic components to drive protein transport through the Golgi complex.
作者信息
Dunphy W G, Pfeffer S R, Clary D O, Wattenberg B W, Glick B S, Rothman J E
出版信息
Proc Natl Acad Sci U S A. 1986 Mar;83(6):1622-6. doi: 10.1073/pnas.83.6.1622.
Abstract
Vesicular transport between successive compartments of the mammalian Golgi apparatus has recently been reconstituted in a cell-free system. In addition to ATP, transport requires both membrane-bound and cytosolic proteins. Here we report that the cytosol fraction from yeast will efficiently substitute for mammalian cytosol. Mammalian cytosol contains several distinct transport factors, which we have distinguished on the basis of gel filtration and ion-exchange chromatography. Yeast cytosol appears to contain the same collection of transport factors. Resolved cytosol factors from yeast and mammals complement each other in a synergistic manner. These findings suggest that the molecular mechanisms of intracellular protein transport have been conserved throughout evolution. Moreover, this hybrid cell-free system will enable the application of yeast genetics to the identification and isolation of cytosolic proteins that sustain intracellular protein transport.
摘要
最近，在无细胞系统中重建了哺乳动物高尔基体连续区室之间的囊泡运输。除ATP外，运输还需要膜结合蛋白和胞质蛋白。在此，我们报告酵母的胞质溶胶部分可有效替代哺乳动物的胞质溶胶。哺乳动物胞质溶胶包含几种不同的运输因子，我们已通过凝胶过滤和离子交换色谱法对其进行了区分。酵母胞质溶胶似乎含有相同的运输因子组合。来自酵母和哺乳动物的已解析胞质溶胶因子以协同方式相互补充。这些发现表明，细胞内蛋白质运输的分子机制在整个进化过程中一直得到保留。此外，这种无细胞杂交系统将使酵母遗传学能够应用于鉴定和分离维持细胞内蛋白质运输的胞质蛋白。
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本文引用的文献
1
The Golgi apparatus (complex)-(1954-1981)-from artifact to center stage.高尔基体（复合体）-（1954年-1981年）-从人为现象到核心地位。
J Cell Biol. 1981 Dec;91(3 Pt 2):77s-103s. doi: 10.1083/jcb.91.3.77s.
2
Order of events in the yeast secretory pathway.酵母分泌途径中的事件顺序。
Cell. 1981 Aug;25(2):461-9. doi: 10.1016/0092-8674(81)90064-7.
3
Compartmentalized assembly of oligosaccharides on exported glycoproteins in yeast.酵母中输出糖蛋白上寡糖的区室化组装
Cell. 1981 Aug;25(2):451-60. doi: 10.1016/0092-8674(81)90063-5.
4
Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway.酵母分泌途径中翻译后事件所需的23个互补组的鉴定。
Cell. 1980 Aug;21(1):205-15. doi: 10.1016/0092-8674(80)90128-2.
5
Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole.酵母分泌途径的早期阶段是羧肽酶Y转运至液泡所必需的。
Cell. 1982 Sep;30(2):439-48. doi: 10.1016/0092-8674(82)90241-0.
6
Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine.通过 N-乙酰葡糖胺的偶联掺入来测定高尔基体连续区室之间蛋白质转运的重建。
Cell. 1984 Dec;39(2 Pt 1):405-16. doi: 10.1016/0092-8674(84)90019-9.
7
Transport of vesicular stomatitis virus glycoprotein in a cell-free extract.水泡性口炎病毒糖蛋白在无细胞提取物中的运输
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3870-4. doi: 10.1073/pnas.77.7.3870.
8
A simplified procedure for synthesizing large quantities of highly purified uridine [beta-32P]diphospho-N-acetylglucosamine.一种合成大量高纯度尿苷[β-32P]二磷酸-N-乙酰葡糖胺的简化方法。
Anal Biochem. 1984 Jul;140(1):264-9. doi: 10.1016/0003-2697(84)90163-5.
9
Dissociation of clathrin coats coupled to the hydrolysis of ATP: role of an uncoating ATPase.网格蛋白包被的解离与ATP水解偶联：一种去包被ATP酶的作用
J Cell Biol. 1984 Aug;99(2):734-41. doi: 10.1083/jcb.99.2.734.
10
Sequential intermediates in the pathway of intercompartmental transport in a cell-free system.无细胞体系中隔室间运输途径的连续中间体。
Cell. 1984 Dec;39(3 Pt 2):525-36. doi: 10.1016/0092-8674(84)90459-8.
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