一种合成大量高纯度尿苷[β-32P]二磷酸-N-乙酰葡糖胺的简化方法。
A simplified procedure for synthesizing large quantities of highly purified uridine [beta-32P]diphospho-N-acetylglucosamine.
作者信息
Lang L, Kornfeld S
出版信息
Anal Biochem. 1984 Jul;140(1):264-9. doi: 10.1016/0003-2697(84)90163-5.
PMID:6237594
Abstract
A simplified procedure for the synthesis of [beta-32P]UDP-N-acetylglucosamine is described. This novel method utilizes commercially available enzymes, chemical acetylation, and preparative thin-layer chromatography and can be used to synthesize millicurie quantities of the sugar nucleotide at an extremely high specific activity and purity.
摘要
本文描述了一种合成[β-32P]UDP-N-乙酰葡糖胺的简化方法。这种新方法利用市售酶、化学乙酰化和制备型薄层色谱，可用于合成毫居里数量的具有极高比活度和纯度的糖核苷酸。
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