Division of Endocrinology, Metabolism and Diabetes, Department of Medicine, University of Colorado Anschutz Medical Campus, 12801 E. 17th Ave, Mail Stop 8106, Aurora, CO, 80045, USA.
Division of Geriatric Medicine, University of Colorado Anschutz Medical Campus, and Eastern Colorado VA Geriatric, Research, Education, and Clinical Center (GRECC), Aurora, CO, USA.
Calcif Tissue Int. 2022 Jun;110(6):712-722. doi: 10.1007/s00223-022-00950-8. Epub 2022 Feb 8.
The goal of this study was to determine the bone turnover marker (BTM) response to insufficient and subsequent recovery sleep, independent of changes in posture, body weight, and physical activity.
Healthy men (N = 12) who habitually slept 7-9 h/night were admitted to an inpatient sleep laboratory for a baseline 8 h/night sleep opportunity followed by six nights of insufficient sleep (5 h/night). Diet, physical activity, and posture were controlled. Serum markers of bone formation (osteocalcin, PINP) and resorption (β-CTX) were obtained over 24 h at baseline and on the last night of sleep restriction, and on fasted samples obtained daily while inpatient and five times after discharge over 3 weeks. Maximum likelihood estimates in a repeated measures model were used to assess the effect of insufficient and subsequent recovery sleep on BTM levels.
There was no statistically or clinically significant change in PINP (p = 0.53), osteocalcin (p = 0.66), or β-CTX (p = 0.10) in response to six nights of insufficient sleep. There were no significant changes in BTMs from the inpatient stay through 3 weeks of recovery sleep (all p [Formula: see text] 0.63). On average, body weight was stable during the inpatient stay (Δweight = - 0.55 ± 0.91 kg, p = 0.06).
No significant changes in serum BTMs were observed after six nights of insufficient or subsequent recovery sleep in young healthy men. Changes in weight and physical activity may be required to observe significant BTM change in response to sleep and circadian disruptions. Clinical Trials Registration Registered at ClinicalTrials.gov (NCT03733483) on November 7, 2018.
本研究旨在确定骨转换标志物（BTM）对睡眠不足及随后恢复睡眠的反应，而不考虑体位、体重和体力活动的变化。
12 名习惯性每晚睡眠 7-9 小时的健康男性入住住院睡眠实验室，有 8 小时的基础睡眠时间，随后进行 6 晚的睡眠不足（每晚 5 小时）。控制饮食、体力活动和体位。在基线和睡眠限制的最后一晚，以及住院期间每天空腹和出院后 3 周内 5 次采集 24 小时血清标志物骨形成（骨钙素、PINP）和骨吸收（β-CTX）。采用重复测量模型的最大似然估计评估睡眠不足和随后恢复睡眠对 BTM 水平的影响。
6 晚睡眠不足时，PINP（p=0.53）、骨钙素（p=0.66）或β-CTX（p=0.10）均无统计学或临床显著变化。从住院到恢复睡眠 3 周，BTM 无明显变化（均 p[公式：见正文]0.63）。住院期间体重基本稳定（Δ体重=-0.55±0.91kg，p=0.06）。
在年轻健康男性中，6 晚睡眠不足或随后恢复睡眠后，血清 BTM 无明显变化。体重和体力活动的变化可能需要观察到睡眠和昼夜节律紊乱对 BTM 变化的显著影响。
临床试验注册 2018 年 11 月 7 日在 ClinicalTrials.gov（NCT03733483）注册。
