Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL, Canada.
Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL, Canada.
Gene. 2022 May 5;821:146211. doi: 10.1016/j.gene.2022.146211. Epub 2022 Feb 5.
B cell acute lymphoblastic leukemia (B-ALL) is the most prevalent pediatric cancer. MicroRNAs (miRNAs) are 18-22nt non-coding transcripts shown to be essential for the development of many cancers. While some miRNAs are reportedly expressed differentially between healthy and B-ALL, no studies have reported a consensus miRNA signature. Therefore, we performed a reanalysis of five miRNA datasets to identify differentially expressed miRNAs (DEmiRs) and a meta-analysis of previously identified DEmiRs from 25 studies. Overall, the re-analysis showed that the DEmiR data clustered by platform and not by disease state. The meta-analysis also did not reveal a consensus miRNA signature as there were many miRNAs upregulated in some studies and downregulated in others. However, eight promising miRNAs (miR-181b, miR-128b, miR-181a, miR-128, miR-128a, miR-181c, miR-155, miR-142-3p, and miR-451) were identified from the meta-analysis, which could be the basis of future investigations. These analyses reveal that standardization of miRNA isolation and analysis is needed in B-ALL to enable cross-study comparisons and identify a consensus signature.
B 细胞急性淋巴细胞白血病 (B-ALL) 是最常见的儿科癌症。microRNAs (miRNAs) 是 18-22nt 的非编码转录物，被证明对许多癌症的发展至关重要。虽然有报道称一些 miRNAs 在健康人和 B-ALL 之间表达差异，但没有研究报道一致的 miRNA 特征。因此，我们对五个 miRNA 数据集进行了重新分析，以确定差异表达的 miRNAs (DEmiRs)，并对 25 项研究中之前确定的 DEmiRs 进行了荟萃分析。总体而言，重新分析表明，DEmiR 数据按平台而不是疾病状态聚类。荟萃分析也没有揭示出一致的 miRNA 特征，因为在一些研究中上调，而在另一些研究中下调。然而，从荟萃分析中确定了八个有前途的 miRNAs（miR-181b、miR-128b、miR-181a、miR-128、miR-128a、miR-181c、miR-155、miR-142-3p 和 miR-451），它们可能是未来研究的基础。这些分析表明，B-ALL 中需要 miRNA 分离和分析的标准化，以实现跨研究比较和确定一致的特征。
