蜜瓜苷 V 对细颗粒物引起的猪肺泡巨噬细胞炎症具有抗炎作用。
Mogroside V exerts anti-inflammatory effects on fine particulate matter-induced inflammation in porcine alveolar macrophages.
机构信息
Research Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, China; National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, China.
Research Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, China; National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, China.
出版信息
Toxicol In Vitro. 2022 Apr;80:105326. doi: 10.1016/j.tiv.2022.105326. Epub 2022 Feb 5.
Mogroside V is the main bioactive component of Siraitia grosvenorii (Swingle), and has a potential anti-inflammatory function. However, the effect of mogroside V on fine particulate matter (PM)-induced inflammation has not been reported. In the present study, the biological effect of mogroside V on inflammation was investigated in PM- treated porcine alveolar macrophages (3D4/21). The results showed that mogroside V significantly inhibited PM-induced nitric oxide (NO) production and rescued the arginase activity inhibited by PM. In the presence of mogroside V, the upregulation of IL-18, TNF-α and COX-2 by PM in 3D4/21 cells was inhibited. Mogroside V attenuated PM-induced phosphorylation of NF-κB p65 and the expression of NLRP3. Mogroside V reduced intracellular ROS levels induced by PM. In the transcriptomic analysis, inflammation-related genes in 3D4/21 cells were not significantly affected after treatment with mogroside V. These results indicated that mogroside V can alleviate the inflammatory response of porcine alveolar macrophages induced by PM from pig house and that mogroside V may play the role through the antioxidant function of eliminating ROS. Mogroside V has a clear anti-inflammatory function in the presence of inflammation.
罗汉果苷 V 是罗汉果（Swingle）的主要生物活性成分，具有潜在的抗炎功能。然而，罗汉果苷 V 对细颗粒物（PM）诱导的炎症的影响尚未有报道。在本研究中，研究了罗汉果苷 V 对 PM 处理的猪肺泡巨噬细胞（3D4/21）炎症的生物学效应。结果表明，罗汉果苷 V 显著抑制 PM 诱导的一氧化氮（NO）产生，并挽救了 PM 抑制的精氨酸酶活性。在罗汉果苷 V 存在的情况下，PM 诱导的 3D4/21 细胞中白细胞介素-18（IL-18）、肿瘤坏死因子-α（TNF-α）和环氧化酶-2（COX-2）的表达上调被抑制。罗汉果苷 V 减弱了 PM 诱导的 NF-κB p65 的磷酸化和 NLRP3 的表达。罗汉果苷 V 降低了 PM 诱导的细胞内 ROS 水平。在转录组分析中，用罗汉果苷 V 处理后，3D4/21 细胞中的炎症相关基因没有明显变化。这些结果表明，罗汉果苷 V 可以减轻猪舍 PM 诱导的猪肺泡巨噬细胞的炎症反应，罗汉果苷 V 可能通过消除 ROS 的抗氧化功能发挥作用。在存在炎症的情况下，罗汉果苷 V 具有明确的抗炎功能。
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