State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Bioresour Technol. 2022 Mar;348:126758. doi: 10.1016/j.biortech.2022.126758. Epub 2022 Feb 5.
Cell self-flocculation endows yeast strains with improved environmental stress tolerance that benefits bioproduction. Exploration of the metabolic and regulatory network differences between the flocculating and non-flocculating cells is conducive to developing strains with satisfactory fermentation efficiency. In this work, integrated analyses of transcriptome, proteome, and phosphoproteome were performed using flocculating yeast Saccharomyces cerevisiae SPSC01 and its non-flocculating mutant grown under acetic acid stress, and the results revealed prominent changes in protein kinases. Overexpressing the mitogen-activated protein kinase Hog1 upregulated by flocculation led to reduced ROS accumulation and increased glutathione peroxidase activity, leading to improved ethanol production under stress. Among the seven genes encoding protein kinases that were tested, AKL1 showed the best performance when overexpressed, achieving higher ethanol productivity in both corncob hydrolysate and simulated corn stover hydrolysate. These results provide alternative strategies for improving cellulosic ethanol production by engineering key protein kinases in S. cerevisiae.
细胞自絮凝赋予酵母菌株更好的环境胁迫耐受性，有利于生物生产。探索絮凝和非絮凝细胞之间的代谢和调控网络差异，有利于开发具有满意发酵效率的菌株。在这项工作中，使用絮凝酵母酿酒酵母 SPSC01 及其在乙酸胁迫下生长的非絮凝突变体进行了转录组、蛋白质组和磷酸蛋白质组的综合分析，结果表明蛋白激酶发生了显著变化。絮凝诱导的丝裂原活化蛋白激酶 Hog1 的过表达导致 ROS 积累减少和谷胱甘肽过氧化物酶活性增加，从而在应激下提高了乙醇产量。在测试的七个编码蛋白激酶的基因中，AKL1 的过表达效果最好，在玉米芯水解物和模拟玉米秸秆水解物中的乙醇产量都更高。这些结果为通过工程酿酒酵母中的关键蛋白激酶来提高纤维素乙醇生产提供了替代策略。
