Shang Jixiang, Zhang Yanchao, Xu Zongjun, Zhang Shouqing, Sun Zhongtao, Zheng Minggang
First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, China.
College of Life Science, Shandong Agricultural University, Tai'an, 271018, China.
Biotechnol Lett. 2025 Jul 22;47(4):80. doi: 10.1007/s10529-024-03552-6.
With the continuous advancement of technologies such as microbial cultivation, DNA sequencing, bioinformatics, and genetic engineering, in vivo mutagenesis methods based on perturbation factors are now widely utilized. We identified a RecJ enzyme (BaRecJ) with endonuclease and exonuclease activities from Bacillus alcalophilus, and established a broad-spectrum mutagenic method based on the endonuclease and exonuclease activities of BaRecJ. The BaRecJ mutagenesis method was applied to S cerevisiae to enhance its ethanol or acetic acid tolerance, resulting in mutant strains with improved fermentation performance. Genomic resequencing analysis summarized genes possibly associated with the tolerance of mutants. BaRecJ mutagenesis method not only holds immense potential in microbial mutagenesis breeding and adaptive evolution but also, when coupled with genomic resequencing, allows for the rapid identification of candidate genetic loci associated with specific traits.
随着微生物培养、DNA测序、生物信息学和基因工程等技术的不断进步，基于扰动因子的体内诱变方法如今得到了广泛应用。我们从嗜碱芽孢杆菌中鉴定出一种具有内切酶和外切酶活性的RecJ酶（BaRecJ），并基于BaRecJ的内切酶和外切酶活性建立了一种广谱诱变方法。将BaRecJ诱变方法应用于酿酒酵母以提高其乙醇或乙酸耐受性，从而获得了发酵性能得到改善的突变菌株。基因组重测序分析总结了可能与突变体耐受性相关的基因。BaRecJ诱变方法不仅在微生物诱变育种和适应性进化方面具有巨大潜力，而且与基因组重测序相结合时，还能快速鉴定与特定性状相关的候选基因位点。
