Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Shandong, 271018, PR China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, Shandong, PR China.
Laboratory of Zoonoses, China Animal Health and Epidemiology Center, Qingdao, Shandong, 266032, PR China.
Poult Sci. 2022 Apr;101(4):101710. doi: 10.1016/j.psj.2022.101710. Epub 2022 Jan 10.
Animal-derived Proteus mirabilis (P. mirabilis) is an important food-borne zoonotic bacillus and widely exists in the broiler-breeding industry. The present study was designed to explore the P. mirabilis prevalence and antimicrobial resistance characteristics in 6 conventional broiler-fattening farms in Shandong Province, China. The overall isolation rate of P. mirabilis was 7.07% (50/707). Antimicrobial resistance was very common in the P. mirabilis isolated from these farms and varied for different antibacterial drugs, with chloramphenicol, ciprofloxacin, and trimethoprim-sulfamethoxazole having the highest resistance rate (98%) and aztreonam the lowest (0%). Multidrug resistance was as high as 100%. The majority of the MDR isolates were resistant to between 9 and 12 of the antibiotics, with these accounting for 76% (38/50) of multidrug resistant strains. These P. mirabilis isolates carried 24 drug-resistance genes in 6 types, with stcM having the highest rate (96%) and cmlA, bla, and qnrC the lowest (2%). Superdrug resistance gene bla was found in 10% (5/50) of isolates from poultry farms in Shandong. All the P. mirabilis isolates carried at least 6 virulence genes, with 100% detection rates of the ireA and hpmA genes. Our study revealed that the P. mirabilis strains isolated in the Shandong area all showed the MDR phenotype and the poultry-derived carbapenem-resistant MDR P. mirabilis strains may pose a potential risk to humans. Surveillance findings presented herein will be conducive to our understanding of the prevalence and characteristics of carbapenem-resistant P. mirabilis strains in Shandong, China.
动物源奇异变形杆菌（P. mirabilis）是一种重要的食源性人畜共患病杆菌，广泛存在于肉鸡养殖行业。本研究旨在探讨山东省 6 个常规肉鸡养殖场奇异变形杆菌的流行情况和耐药特征。奇异变形杆菌的总分离率为 7.07%（50/707）。这些来自养殖场的奇异变形杆菌对抗菌药物的耐药性非常普遍，不同抗菌药物的耐药率也不同，氯霉素、环丙沙星和甲氧苄啶-磺胺甲恶唑的耐药率最高（98%），而氨曲南的耐药率最低（0%）。多重耐药率高达 100%。大多数 MDR 分离株对 9 到 12 种抗生素耐药，其中 76%（38/50）的 MDR 分离株对 12 种抗生素耐药，这些分离株携带 6 种 24 种耐药基因，其中 stcM 的携带率最高（96%），cmlA、bla 和 qnrC 的携带率最低（2%）。在来自山东家禽养殖场的 10%（5/50）分离株中发现了超级耐药基因 bla。所有奇异变形杆菌分离株均携带至少 6 种毒力基因，ireA 和 hpmA 基因的检测率为 100%。我们的研究表明，从山东地区分离的奇异变形杆菌菌株均表现出 MDR 表型，禽源碳青霉烯类耐药 MDR 奇异变形杆菌菌株可能对人类构成潜在威胁。本研究提供的监测结果将有助于我们了解中国山东省碳青霉烯类耐药奇异变形杆菌菌株的流行情况和特征。
