Department of Veterinary Public Health, College of Veterinary Medicine & Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Taian City, 271018, Shandong, PR China.
Department of Internal Medicine-Cardiovascular, The Affiliated Tai'an City Central Hospital of Qingdao University, Taian City, 271000, Shandong, PR China.
Poult Sci. 2023 Mar;102(3):102483. doi: 10.1016/j.psj.2023.102483. Epub 2023 Jan 7.
Animal-derived Enterobacteriaceae bacteria such as Escherichia coli (E. coli), Proteus mirabilis (P. mirabilis), and Klebsiella pneumoniae (K. pneumoniae) are important food-borne zoonotic bacilli that exist widely in the broiler-breeding industry. Although carbapenem antibiotics are considered to be the last line of defense against multidrug-resistant bacteria, carbapenem-resistant Enterobacteriaceae (CRE) break through them. In our study, we therefore, examined the prevalence of CRE and characteristics of antimicrobial resistance in 6 conventional broiler-fattening farms in Shandong Province, China. Our study revealed isolation rates of 3.57% (6/168) for carbapenem-resistant E. coli, 10% (5/50) for carbapenem-resistant P. mirabilis, and 3.03% (1/33) for carbapenem-resistant K. pneumoniae. All 12 CRE bacterial strains showed varying degrees of resistance to 27 antibiotics in 8 classes and were multidrug-resistant. The rate of the strains containing bla genes, at 91.67% (11/12), was especially high. Among other results, the carrying rate of integrons in CRE bacteria was 91.67% (11/12), and 2 strains carried both class I and class II integrons, which accelerated the lateral transmission of resistant bacteria. Our first-ever finding of the 3 CRE bacteria E. coli, P. mirabilis, and K. pneumoniae on the same broiler farm suggests that poultry-derived CRE strains may pose a risk to humans. Moreover, our findings from surveillance can inform current understandings of the prevalence and characteristics of multidrug-resistant CRE in Shandong Province and, in turn, help to curb threats to food safety and public health and better prevent and control infectious zoonotic diseases.
动物源肠杆菌科细菌，如大肠杆菌（E. coli）、奇异变形杆菌（P. mirabilis）和肺炎克雷伯菌（K. pneumoniae），是广泛存在于肉鸡养殖行业的重要食源性人畜共患病杆菌。虽然碳青霉烯类抗生素被认为是治疗多药耐药菌的最后一道防线，但碳青霉烯类耐药肠杆菌科（CRE）却突破了这一防线。在我们的研究中，我们因此检测了山东省 6 个常规肉鸡养殖场 CRE 的流行情况和抗菌药物耐药特征。我们的研究显示，耐碳青霉烯类大肠杆菌的分离率为 3.57%（6/168），耐碳青霉烯类奇异变形杆菌为 10%（5/50），耐碳青霉烯类肺炎克雷伯菌为 3.03%（1/33）。12 株 CRE 细菌对 8 类 27 种抗生素表现出不同程度的耐药性，均为多重耐药菌。携带 bla 基因的菌株比例高达 91.67%（11/12）。其他结果还包括，CRE 细菌中整合子的携带率为 91.67%（11/12），有 2 株携带 I 类和 II 类整合子，这加速了耐药菌的横向传播。我们首次在同一肉鸡养殖场发现 3 种 CRE 细菌，即大肠杆菌、奇异变形杆菌和肺炎克雷伯菌，这表明禽源 CRE 菌株可能对人类构成威胁。此外，我们的监测结果有助于了解山东省多药耐药 CRE 的流行情况和特征，从而有助于遏制对食品安全和公众健康的威胁，并更好地预防和控制传染性人畜共患病。
