Department of Periodontology, Faculty of Dentistry, Uşak University, Uşak, Turkey.
Department of Periodontogy, Faculty of Dentistry, Süleyman Demirel University, Isparta, Turkey.
Biotech Histochem. 2022 Nov;97(8):567-575. doi: 10.1080/10520295.2022.2036370. Epub 2022 Feb 9.
We investigated the effects of caffeic acid phenethyl ester (CAPE) and low-dose doxycycline (LDD) on sclerostin and bone morphogenic protein (BMP)-2 expression in experimental periodontitis. We used male rats in groups as follows: control group (C), periodontitis + CAPE group (PC), periodontitis + LDD group (PD), periodontitis + LDD + CAPE group (PCD) and periodontitis group (P). We administered 10 µmol/kg/day CAPE by an intraperitoneal (i.p.) injection and 10 mg/kg/day LDD by oral gavage. Histopathological changes among groups were evaluated and compared. Sclerostin and BMP-2 expression was analyzed using immunohistochemistry. LDD and/or CAPE treatment ameliorated pathology. The highest sclerostin and lowest BMP-2 expressions were found in P group. Group PC exhibited the highest BMP-2 expression scores and the most significant improvement among the treatment groups. The lowest sclerostin expression was observed in the PD group. We found that preventing sclerostin activity may be a useful treatment alternative for bone resorption, especially in cases of periodontitis and peri-implantitis. We found that CAPE and/or LDD may act as anti-sclerostin agents.
我们研究了咖啡酸苯乙酯（CAPE）和低剂量强力霉素（LDD）对实验性牙周炎中骨硬化蛋白（sclerostin）和骨形态发生蛋白-2（BMP-2）表达的影响。我们将雄性大鼠分为以下几组：对照组（C）、牙周炎+CAPE 组（PC）、牙周炎+LDD 组（PD）、牙周炎+LDD+CAPE 组（PCD）和牙周炎组（P）。我们通过腹腔内（i.p.）注射给予 10 μmol/kg/天的 CAPE 和口服给予 10 mg/kg/天的 LDD。评估并比较各组的组织病理学变化。采用免疫组织化学法分析 sclerostin 和 BMP-2 的表达。LDD 和/或 CAPE 治疗改善了病理学。P 组的 sclerostin 表达最高，BMP-2 表达最低。PC 组的 BMP-2 表达评分最高，在治疗组中改善最显著。PD 组的 sclerostin 表达最低。我们发现抑制 sclerostin 活性可能是一种治疗骨吸收的有效方法，尤其是在牙周炎和种植体周围炎的情况下。我们发现 CAPE 和/或 LDD 可能具有抗 sclerostin 作用。
