Uşak University, Faculty of Dentistry, Department of Periodontology, Uşak, Turkey.
Süleyman Demirel University, Faculty of Dentistry, Department of Periodontology, Isparta, Turkey.
Arch Oral Biol. 2017 Sep;81:61-68. doi: 10.1016/j.archoralbio.2017.04.017. Epub 2017 Apr 21.
Host modulation therapies (anti-inflammatory drugs, bone-stimulating agents, anti-proteinase etc.) target the inhibition or stabilization of tissue breakdown. The aim of the present study was to evaluate the effects of caffeic acid phenethyl ester (CAPE) and/or low dose doxycycline (LDD) administrations on alveolar bone loss (ABL), serum cytokines and gingival apoptosis, as well as the levels of oxidants and anti-oxidants in rats with ligature-induced periodontitis.
The animals were randomly divided into five groups: Group C (periodontally healthy), Group PC (Periodontitis+CAPE), Group PD (Periodontitis+LDD), Group PCD (Periodontitis+CAPE+LDD), Group P (Periodontitis). Experimental periodontitis was induced for 14days. Levels of ABL, and the serum cytokines, interleukin (IL)-1 β, IL-6, tumor necrosis factor-α (TNF-α) and IL-10 were assessed as were the levels of the oxidants and anti-oxidants, malondialdehyde (MDA), glutathione (GSH) and glutathione peroxidase (GSH-Px), and levels of gingival apoptosis.
The lowest ABL levels was evident in the PC group, among the experimental groups. There was also less inflammatory infiltration in the PC group than the PD group. IL-1β, IL-6, and IL-10 were lower in the PC group and higher in the P group in comparison to the levels in the other experiment groups. TNF-α levels in the PD group were higher than levels in the PC and PCD groups. The PC and PCD groups did not differ from the C group in regard to MDA levels. The highest GSH-Px level was found in the PC group. Gingival apoptosis in the PC group was not only lower than the PD and PCD groups, but also lower than in the C group.
The present study suggests that CAPE has more anti-inflammatory, anti-oxidant and anti-apoptotic effects than LDD, with no additive benefits of a CAPE+LDD combination being evident in rats with periodontitis.
宿主调节疗法（抗炎药物、骨刺激剂、抗蛋白酶等）旨在抑制或稳定组织破坏。本研究旨在评估咖啡酸苯乙酯（CAPE）和/或低剂量强力霉素（LDD）给药对结扎诱导牙周炎大鼠牙槽骨丢失（ABL）、血清细胞因子和牙龈凋亡以及氧化剂和抗氧化剂水平的影响。
动物随机分为五组：C 组（牙周健康）、PC 组（牙周炎+CAPE）、PD 组（牙周炎+LDD）、PCD 组（牙周炎+CAPE+LDD）和 P 组（牙周炎）。实验性牙周炎诱导 14 天。评估 ABL 水平以及血清细胞因子白细胞介素（IL）-1β、IL-6、肿瘤坏死因子-α（TNF-α）和 IL-10 水平，评估氧化剂和抗氧化剂水平，丙二醛（MDA）、谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GSH-Px）水平，以及牙龈凋亡水平。
在实验组中，PC 组的 ABL 水平最低。与 PD 组相比，PC 组的炎症浸润也较少。与其他实验组相比，PC 组的 IL-1β、IL-6 和 IL-10 水平较低，而 P 组的水平较高。PD 组的 TNF-α水平高于 PC 和 PCD 组。PC 和 PCD 组的 MDA 水平与 C 组无差异。PC 组的 GSH-Px 水平最高。PC 组的牙龈凋亡不仅低于 PD 和 PCD 组，而且低于 C 组。
本研究表明，CAPE 比 LDD 具有更强的抗炎、抗氧化和抗凋亡作用，而 CAPE+LDD 联合使用在牙周炎大鼠中没有显示出额外的益处。
